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      Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease Translated title: Discordâncias e consequências de resultados de provas de hemaglutinação indireta, imunofluorescência indireta e Elisa para o diagnóstico da doença de Chagas

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          Abstract

          Using the indirect hemagglutination (IH), indirect immunofluorescence (IIF) and enzyme linked immunosorbent assay (ELISA) tests for the diagnosis of Chagas disease, 4000 serum samples were examined. This study was conducted with different purposes: clinical interest, research support and parasitological monitoring of those patients with Chagas disease who were treated with heart transplantations. The tests occurred without patient selection and in accordance with the medical requests. The results showed discrepancies and brought about several questions, considering the different results that all three methods showed when considered together. What was found brought about concerns and we suggest the adoption of different measures, aiming to avoid these mismatches in the context of this disease.

          Translated abstract

          Com as provas de hemaglutinação indireta (HI), imunofluorescência indireta (IFI) e Enzyme Linked Immunosorbent Assay (ELISA), para diagnóstico da doença de Chagas, foram examinadas concomitantemente 4000 amostras de soro, com diferentes finalidades, tais como interesse clínico, apoio a pesquisas e acompanhamento parasitológico de pacientes com tal moléstia tratados por meio de transplante de coração. Os testes ocorreram, sem seleção e conforme as solicitações, em Laboratório que essencialmente prestou colaboração. Os resultados mostraram discordâncias, inclusive motivadoras de dúvidas, considerando especificamente o revelado pelos três métodos em conjunto. O que ficou verificado suscita preocupações e sugere a adoção de medidas aptas a evitar essas inadequações no contexto da parasitose.

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          Most cited references13

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          Introdução às técnicas de imunofluorescência.

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            Introdução às técnicas de imunofluorescência

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              • Article: not found

              Risk of exposure to Chagas' disease among seroreactive Brazilian blood donors

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Journal
                rimtsp
                Revista do Instituto de Medicina Tropical de São Paulo
                Rev. Inst. Med. trop. S. Paulo
                Instituto de Medicina Tropical (São Paulo )
                1678-9946
                June 2012
                : 54
                : 3
                : 141-144
                Affiliations
                [1 ] Universidade de São Paulo Brazil
                Article
                S0036-46652012000300005
                10.1590/S0036-46652012000300005
                a83eca1a-7cc3-4af4-b9f8-2b36ce49f350

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0036-4665&lng=en
                Categories
                TROPICAL MEDICINE

                Infectious disease & Microbiology
                Chagas disease,Serological diagnosis,Discrepancies,Consequences

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