G-protein-coupled receptors (GPCRs) represent the major protein family for cellular
modulation in mammals. Therefore, various strategies have been developed to analyze
the function of GPCRs involving pharmaco- and optogenetic approaches [1, 2]. However,
a tool that combines precise control of the activation and deactivation of GPCR pathways
and/or neuronal firing with limited phototoxicity is still missing. We compared the
biophysical properties and optogenetic application of a human and a mouse melanopsin
variant (hOpn4L and mOpn4L) on the control of Gi/o and Gq pathways in heterologous
expression systems and mouse brain. We found that GPCR pathways can be switched on/off
by blue/yellow light. The proteins differ in their kinetics and wavelength dependence
to activate and deactivate G protein pathways. Whereas mOpn4L is maximally activated
by very short light pulses, leading to sustained G protein activation, G protein responses
of hOpn4L need longer light pulses to be activated and decline in amplitude. Based
on the different biophysical properties, brief light activation of mOpn4L is sufficient
to induce sustained neuronal firing in cerebellar Purkinje cells (PC), whereas brief
light activation of hOpn4L induces AP firing, which declines in frequency over time.
Most importantly, mOpn4L-induced sustained firing can be switched off by yellow light.
Based on the biophysical properties, hOpn4L and mOpn4L represent the first GPCR optogenetic
tools, which can be used to switch GPCR pathways/neuronal firing on an off with temporal
precision and limited phototoxicity. We suggest to name these tools moMo and huMo
for future optogenetic applications.