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      Taxonomic synopsis of invasive and native Spartina (Poaceae, Chloridoideae) in the Pacific Northwest (British Columbia, Washington and Oregon), including the first report of Spartina ×townsendii for British Columbia, Canada

      research-article
      1
      PhytoKeys
      Pensoft Publishers
      invasive grasses, voucher specimens, coastal habitats, Spartina, grass taxonomy, species identification

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          Abstract

          Abstract

          Five species of the grass genus Spartina are invading salt marshes along the Pacific coast of North America, of which three have been documented in British Columbia, Canada, in only the last decade. A taxonomic synopsis of the two native ( Spartina gracilis , Spartina pectinata ) and five introduced Spartina taxa ( Spartina anglica , Spartina alterniflora , Spartina densiflora , Spartina patens , Spartina ×townsendii ) in the Pacific Northwest is presented to facilitate their identification, including nomenclature, a new taxonomic key, new descriptions for a subset of taxa, and representative specimens. Spartina ×townsendii is newly reported for the flora of British Columbia. The non-coastal species Spartina pectinata is reported from an urban site in British Columbia, the first confirmed report of the taxon for the province. Lectotypes are newly designated for Spartina anglica C.E. Hubb., Spartina maritima  subvar.  fallax St.-Yves, and Spartina cynosuroides f. major St.-Yves.

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          Genetic and epigenetic consequences of recent hybridization and polyploidy in Spartina (Poaceae).

          To study the consequences of hybridization and genome duplication on polyploid genome evolution and adaptation, we used independently formed hybrids (Spartina x townsendii and Spartina x neyrautii) that originated from natural crosses between Spartina alterniflora, an American introduced species, and the European native Spartina maritima. The hybrid from England, S. x townsendii, gave rise to the invasive allopolyploid, salt-marsh species, Spartina anglica. Recent studies indicated that allopolyploid speciation may be associated with rapid genetic and epigenetic changes. To assess this in Spartina, we performed AFLP (amplified fragment length polymorphism) and MSAP (methylation sensitive amplification polymorphism) on young hybrids and the allopolyploid. By comparing the subgenomes in the hybrids and the allopolyploid to the parental species, we inferred structural changes that arose repeatedly in the two independently formed hybrids. Surprisingly, 30% of the parental methylation patterns are altered in the hybrids and the allopolyploid. This high level of epigenetic regulation might explain the morphological plasticity of Spartina anglica and its larger ecological amplitude. Hybridization rather than genome doubling seems to have triggered most of the methylation changes observed in Spartina anglica.
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            Pollen limitation causes an Allee effect in a wind-pollinated invasive grass (Spartina alterniflora).

            It is usually assumed that pollen availability does not limit reproduction in wind-pollinated plants. Little evidence either supporting or contradicting this assumption exists, despite the importance of seed production to population persistence and growth. We investigated the role of pollen limitation in an invasive estuarine grass (Spartina alterniflora), with a manipulative pollen supplementation and exclusion experiment in areas of high population density and at the low-density leading edge of the invasion. We also quantified pollen deposition rates on stigmas and pollen traps along a windward to leeward gradient. We found pollen impoverishment at the low-density leading edge of a large invasion, causing an 8-fold reduction in seed set. We found 9-fold more pollen on stigmas of high-density plants than on those of low-density plants. Pollen deposition rates on stigmas and traps did not increase downwind of low-density plants but did increase downwind of high-density plants and dropped off precipitously across a gap that lacked pollen donors. The delay of appreciable numbers of seed caused by pollen limitation persists for decades until vegetative growth coalesces plants into continuous meadows, and this Allee effect has slowed the rate of spread of the invasion.
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              Retrotransposons and genomic stability in populations of the young allopolyploid species Spartina anglica C.E. Hubbard (Poaceae).

              Spartina x townsendii arose during the end of the 19th century in England by hybridization between the indigenous Spartina maritima and the introduced Spartina alterniflora, native to the eastern seaboard of North America. Duplication of the hybrid genome gave rise to Spartina anglica, a vigorous allopolyploid involved in natural and artificial invasions on several continents. This system allows investigation of the early evolutionary changes that accompany stabilization of new allopolyploid species. Because allopolyploidy may be a genomic shock, eliciting retroelement insertional activity, we examined whether retrotransposons present in the parental species have been activated in the genome of S. anglica. For this purpose we used inter-retrotransposon amplified polymorphism (IRAP) and retrotransposons-microsatellite amplified polymorphism (REMAP) markers, which are multilocus PCR-based methods detecting retrotransposon integration events in the genome. IRAP and REMAP allowed the screening of insertional polymorphisms in populations of S. anglica. The populations are composed mainly of one major multilocus genotype, identical to the first-generation hybrid S. x townsendii. Few new integration sites were encountered in the young allopolyploid genome. We also found strict additivity of the parental subgenomes in the allopolyploid. Both these findings indicate that the genome of S. anglica has not undergone extensive changes since its formation. This contrasts with previous results from the literature, which report rapid structural changes in experimentally resynthesized allopolyploids.
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                Author and article information

                Journal
                PhytoKeys
                PhytoKeys
                PhytoKeys
                PhytoKeys
                Pensoft Publishers
                1314-2011
                1314-2003
                2012
                21 March 2012
                : 10
                : 25-82
                Affiliations
                [1 ]Research & Collections, Canadian Museum of Nature, P.O. Box 3443 Stn. D, Ottawa, Ontario K1P 6P4, Canada
                Author notes
                Corresponding author: Jeffery M. Saarela ( jsaarela@ 123456mus-nature.ca )

                Academic editor: W. John Kress

                Article
                10.3897/phytokeys.10.2734
                3310194
                22461730
                a89c8732-3ebe-487d-bb34-ab8405cd8f4e
                Jeffery M. Saarela

                This is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 20 January 2012
                : 8 March 2012
                Categories
                Article

                Plant science & Botany
                voucher specimens,spartina,species identification,invasive grasses,grass taxonomy,coastal habitats

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