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      Amplicon Sequencing in the Era of Highly-Accurate Long Reads

      ARPHA Conference Abstracts

      Pensoft Publishers

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          Abstract

          An important advance in DNA sequencing has been the development of long-read sequencing technologies that produce sequencing reads of tens to hundreds of kilobases in length. However, these technologies typically have high (~8%) per-base error rates. Recently, an effectively new technology I call highly-accurate long-read sequencing has been developed, that allows for the generation of multi-kilobase reads with extremely high per-base accuracies (>99.9%). I will present and evaluate two such technologies, PacBio HiFi and LoopSeq SLR sequencing, and discuss potential metabarcoding applications of highly-accurate long-read amplicon sequencing in general.

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          Author and article information

          Journal
          ARPHA Conference Abstracts
          ACA
          Pensoft Publishers
          2603-3925
          March 04 2021
          March 04 2021
          : 4
          Article
          10.3897/aca.4.e65405
          © 2021

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