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      Prolactin Receptor: Identification of the Binding Unit by Affinity Labelling and Characterization of Poly- and Monoclonal Antibodies

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          The prolactin receptor localized in rabbit mammary gland membranes has been identified by affinity labelling using covalent cross-linking agents such as a unique protein chain of approximately 32,000 daltons. After partial purification (5,000-fold) of these receptors from mammary gland homogenate, polyclonal antibodies, which specifically and completely inhibit prolactin binding in all organs and in all species studied, were raised. These antibodies possessed prolactin-like biological activity (casein synthesis) on rabbit mammary gland explants. Monoclonal antibodies specifically directed against the binding domain of the receptor were also obtained. These antibodies were more species-specific than the polyclonal antibodies. The most potent (M11O) possessed higher affinity than prolactin for the receptor and could be a very effective tool to elucidate the structure of the receptor and its immunological detection.

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          Author and article information

          Hormone Research in Paediatrics
          S. Karger AG
          28 November 2008
          : 22
          : 3
          : 179-188
          aLaboratoire de Physiologie de la Lactation, INRA-CNRZ, Jouy-en-Josas, France bLaboratory of Molecular Endocrinology, Royal Victoria Hospital, Montréal, Qué., Canada
          180092 Horm Res 1985;22:179–188
          © 1985 S. Karger AG, Basel

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          Pages: 10


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