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      Assay of Corticotrophin Releasing Factor (CRF) in ACTH-Primed ‘Grafted’ Rats

      ,

      Neuroendocrinology

      S. Karger AG

      ACTH, CRF, bioassay, Ectopic hypophyseal grafts

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          Abstract

          An assay for corticotrophin releasing factor (CRF) is described, using plasma corticosterone responses in ACTH-primed ‘grafted’ rats (hypophysectomized rats with multiple ectopic pituitary grafts). Pooled acid extracts of rat stalk median eminences (SME) were used as a local CRF standard. A satisfactory dose response relationship exists for intravenous doses of SME between 0.2 and 1.0 SME and the index of precision ( λ) is approximately 0.2. The ACTH (0.22 mU (1962)/SME) and vasopressin content (<5 mU/SME) of rat hypothalamic extracts make a negligible contribution to the assay responses. No potency difference was found between frozen and freeze-dried extracts of pooled rat SME.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1972
          1972
          19 March 2008
          : 10
          : 2
          : 83-93
          Affiliations
          Neuroendocrinology Research Group, Medical Research Council of New Zealand, Medical School, Dunedin
          Article
          122081 Neuroendocrinology 1972;10:83–93
          10.1159/000122081
          4340091
          © 1972 S. Karger AG, Basel

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          Page count
          Pages: 11
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          Paper

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