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      Effects of Dietary Protein on Eicosanoid Production in Rat Renal Tubules

      ,

      Nephron

      S. Karger AG

      Eicosanoid, Tubule, Cyclooxygenase, Protein, Phospholipase A2 , Phospholipase C

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          Abstract

          The effects of dietary protein on the in vitro production of prostaglandin (PG) E<sub>2</sub>, 6-keto PGF<sub>1α</sub>, and thromboxane B<sub>2</sub> (TxB<sub>2</sub>) were examined in cortical and medullary tubules from rats fed a low (6% casein) or a high (40% casein) protein diet for approximately 8 weeks. PGE<sub>2</sub> was a major eicosanoid produced by rat renal tubules (PGE<sub>2</sub> > TxB<sub>2</sub> > 6-keto PGF<sub>1α</sub>). Medullary tubules produced significantly greater amounts of PGE<sub>2</sub>, 6-keto PGF<sub>1α</sub>, and TxB<sub>2</sub> than cortical tubules. The activity of phospholipase (PL) A<sub>2</sub> and PLC and the levels of cyclooxygenase (COX) were significantly greater in medullary tubules than in cortical tubules. The production of eicosanoids may be increased in medullary versus cortical tubules via the greater activity of these enzymes involved in eicosanoid formation. Although there were no substantial differences in the production of PGE<sub>2</sub>, 6-keto PGF<sub>1α</sub>, and TxB<sub>2</sub> between cortical tubules from rats fed a low- or a high-protein diet, significant increases in the synthesis of the three eicosanoids were observed in medullary tubules from rats fed a high- versus a low-protein diet. The activity of membrane-bound PLA<sub>2</sub> and PLC and the levels of COX were not also substantially different between cortical tubules from rats fed a low- or a high-protein diet. However, medullary tubules had significantly greater activity of membrane-associated PLA<sub>2</sub> and COX in rats fed a high-protein diet than in those fed a low-protein diet. The activity of PLC was comparable in medullary tubules from rats fed a low- or a high-protein diet. We conclude that a high protein intake selectively enhances eicosanoid production in rat medullary tubules probably via activation of the PLA<sub>2</sub>-COX pathway.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1998
          February 1998
          26 January 1998
          : 78
          : 2
          : 179-186
          Affiliations
          Department of Hygiene and Preventive Medicine, Faculty of Medicine, Saitama Medical School, Saitama, Japan
          Article
          44908 Nephron 1998;78:179–186
          10.1159/000044908
          9496735
          © 1998 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Figures: 1, Tables: 3, References: 30, Pages: 8
          Product
          Self URI (application/pdf): https://www.karger.com/Article/Pdf/44908
          Categories
          Original Paper

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