Shengli Zhao 1 , Jonathan T. Ting 1 , 2 , Hisham E. Atallah 2 , Li Qiu 1 , Jie Tan 3 , Bernd Gloss 1 , 4 , George J. Augustine 5 , 6 , 7 , Karl Deisseroth 8 , Minmin Luo 3 , Ann M. Graybiel 2 , Guoping Feng 1 , 2
12 October 2011
Channelrhodopsin-2, ChR2-EYFP, ChR2(H134R), VGAT, ChAT, TPH2, Parvalbumin, Pvalb, electrophysiology, acute brain slice, dorsal raphe nucleus, Purkinje cells, thalamic reticular nucleus, striatal cholinergic neurons, cortical fast-spiking interneurons, hippocampal interneurons, bacterial artificial chromosome, BAC, transgenic mice
Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function, and dysfunction. We used a Bacterial Artificial Chromosome (BAC) transgenic strategy to express Channelrhodopsin2 (ChR2) under the control of cell-type specific promoter elements. We provide a detailed functional characterization of the newly established VGAT-ChR2-EYFP, ChAT-ChR2-EYFP, TPH2-ChR2-EYFP and Pvalb-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action potential firing of GABAergic, cholinergic, serotonergic, and parvalbumin+ neuron subsets using blue light. This resource of cell type-specific ChR2 mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.