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Comparative study on characterization and wound healing potential of goat (Capra hircus) mesenchymal stem cells derived from fetal origin amniotic fluid and adult bone marrow.

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      Abstract

      Caprine amniotic fluid (cAF) and bone marrow cells (cBM) were isolated, expanded and phenotypically characterized by mesenchymal stem cells (MSCs) specific cell surface markers. Both cell types were compared for multilineage differentiation potential by flow cytometry using specific antibodies against lineage specific markers. Furthermore, in vitro expanded cAF-MSCs showed higher expression of trophic factors viz. VEGF and TGF-β1 as compared to cBM-MSCs. Full-skin thickness excisional wounds created on either side of the dorsal midline (thoracolumbar) of New Zealand White rabbits were randomly assigned to subcutaneous injection of either fetal origin cAF-MSCs (n=4) or adult cBM-MSCs (n=4) or sterile PBS (control, n=4). The rate of wound closure was found faster (p<0.05) in cAF-MSCs treated wounds as compared with cBM-MSCs and PBS treated wounds especially on 21st day post-skin excision. Histomorphological examination of the healing tissue showed that wound healing was improved (p<0.05) by greater epithelialization, neovascularization and collagen development in cAF-MSCs as compared to cBM-MSCs and PBS treated wounds.

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      Affiliations
      [1 ] Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; Kerala Veterinary and Animal Sciences University, Kerala, India.
      [2 ] Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; Centre for Genetic Disorders, Institute of Science, Banaras Hindu University, Varanasi, India.
      [3 ] Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; College of Veterinary and Animal Sciences, Durg, India.
      [4 ] Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; Central Drug Research Institute, Lucknow, India.
      [5 ] Division of Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India.
      [6 ] Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India.
      [7 ] Kerala Veterinary and Animal Sciences University, Kerala, India.
      [8 ] Division of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India.
      [9 ] Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India.
      [10 ] Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India. Electronic address: gts553@gmail.com.
      Journal
      Res. Vet. Sci.
      Research in veterinary science
      Elsevier BV
      1532-2661
      0034-5288
      Jun 2017
      : 112
      28135618 S0034-5288(16)30836-0 10.1016/j.rvsc.2016.12.009

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