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Practical HPLC Method Development
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Author(s):
Lloyd R. Snyder
,
Joseph J. Kirkland
,
Joseph L. Glajch
Publication date
(Print):
February 28 1997
Publisher:
John Wiley & Sons, Inc.
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Book
ISBN (Electronic):
9781118592014
ISBN (Print):
9780471007036
Publication date (Print):
February 28 1997
DOI:
10.1002/9781118592014
SO-VID:
aa1d32cc-7360-4661-9718-9cad3573bd57
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Book chapters
pp. i
Front Matter
pp. 1
Getting Started
pp. 21
Basics of Separation
pp. 59
Detection Sensitivity and Selectivity
pp. 100
Sample Preparation
pp. 174
The Column
pp. 233
Non-Ionic Samples: Reversed- and Normal-Phase HPLC
pp. 292
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC
pp. 350
Gradient Elution
pp. 402
Systematic Approach to the Reversed-Phase Separation of Regular Samples
pp. 439
Computer-Assisted Method Development
pp. 479
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds
pp. 537
Chiral Separations
pp. 616
Preparative HPLC Separation
pp. 643
Quantitation (Including Trace Analysis)
pp. 685
Completing the Method: Validation and Transfer
pp. 714
Appendix I: Plate Number and Resolution
pp. 721
Appendix II: Properties of Solvents Used in HPLC
pp. 729
Appendix III: Retention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure
pp. 735
Appendix IV: Preparing Buffered Mobile Phases
pp. 740
Appendix V: Characterizing the Differences Among C8or C18Reversed-Phase Columns from Different Suppliers
pp. 744
Appendix VI: Adjusting Mobile-Phase Water Content for Normal-Phase HPLC
pp. 747
Index
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