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      Repositionable Compounds with Antifungal Activity against Multidrug Resistant Candida auris Identified in the Medicines for Malaria Venture’s Pathogen Box

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      Journal of Fungi
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      Candida auris, antifungals, drug screening, Pathogen Box, repurposing

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          Abstract

          Background. Candida auris has spread rapidly around the world as a causative agent of invasive candidiasis in health care facilities and there is an urgent need to find new options for treating this emerging, often multidrug-resistant pathogen. Methods. We screened the Pathogen Box ® chemical library for inhibitors of C. auris strain 0390, both under planktonic and biofilm growing conditions. Results. The primary screen identified 12 compounds that inhibited at least 60% of biofilm formation or planktonic growth. After confirmatory dose-response assays, iodoquinol and miltefosine were selected as the two main leading repositionable compounds. Iodoquinol displayed potent in vitro inhibitory activity against planktonic C. auris but showed negligible inhibitory activity against biofilms; whereas miltefosine was able to inhibit the growth of C. auris under both planktonic and biofilm-growing conditions. Subsequent experiments confirmed their activity against nine other strains C. auris clinical isolates, irrespective of their susceptibility profiles against conventional antifungals. We extended our studies further to seven different species of Candida, also with similar findings. Conclusion. Both drugs possess broad spectrum of activity against Candida spp., including multiple strains of the emergent C. auris, and may constitute promising repositionable options for the development of novel therapeutics for the treatment of candidiasis.

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          First hospital outbreak of the globally emerging Candida auris in a European hospital

          Background Candida auris is a globally emerging multidrug resistant fungal pathogen causing nosocomial transmission. We report an ongoing outbreak of C. auris in a London cardio-thoracic center between April 2015 and July 2016. This is the first report of C. auris in Europe and the largest outbreak so far. We describe the identification, investigation and implementation of control measures. Methods Data on C. auris case demographics, environmental screening, implementation of infection prevention/control measures, and antifungal susceptibility of patient isolates were prospectively recorded then analysed retrospectively. Speciation of C. auris was performed by MALDI-TOF and typing of outbreak isolates performed by amplified fragment length polymorphism (AFLP). Results This report describes an ongoing outbreak of 50 C. auris cases over the first 16 month (April 2015 to July 2016) within a single Hospital Trust in London. A total of 44 % (n = 22/50) patients developed possible or proven C. auris infection with a candidaemia rate of 18 % (n = 9/50). Environmental sampling showed persistent presence of the yeast around bed space areas. Implementation of strict infection and prevention control measures included: isolation of cases and their contacts, wearing of personal protective clothing by health care workers, screening of patients on affected wards, skin decontamination with chlorhexidine, environmental cleaning with chorine based reagents and hydrogen peroxide vapour. Genotyping with AFLP demonstrated that C. auris isolates from the same geographic region clustered. Conclusion This ongoing outbreak with genotypically closely related C. auris highlights the importance of appropriate species identification and rapid detection of cases in order to contain hospital acquired transmission.
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            Candida auris: A rapidly emerging cause of hospital-acquired multidrug-resistant fungal infections globally

            Candidiasis, which includes both superficial infections and invasive disease, is the most common cause of fungal infection worldwide. Candida bloodstream infections (BSI) cause significant mortality and elicit a major threat to intensive care unit (ICU) patients [1]. The annual global burden of Candida spp. BSIs is about 400,000 cases, with most cases reported from the developed world. Although Candida albicans remains the most frequently isolated Candida species in the clinical setting, in some countries, a marked shift towards species of Candida that have increased resistance to azoles such as fluconazole (FLU), the standard antifungal drug of choice in many countries, and to the recently introduced antifungals known as echinocandins, is reported. Several species of non-albicans Candida, such as C. tropicalis, C. glabrata, and C. parapsilosis, are well-recognized pathogens in BSIs in different geographic locations. More recently, Candida auris, a multidrug-resistant (MDR) yeast that exhibits resistance to FLU and markedly variable susceptibility to other azoles, amphotericin B (AMB), and echinocandins, has globally emerged as a nosocomial pathogen (Fig 1) [2–20]. Alarmingly, in a span of only 7 years, this yeast, which is difficult to treat and displays clonal inter- and intra-hospital transmission, has become widespread across several countries, causing a broad range of healthcare-associated invasive infections [4, 5, 10, 12, 21, 22]. 10.1371/journal.ppat.1006290.g001 Fig 1 A global map depicting rapid emergence of multidrug-resistant clinical Candida auris strains in 5 continents. The value in parentheses denotes the year of report of C. auris from the respective country or state. Why is C. auris often misidentified in the routine microbiology laboratory? In 2009, a novel Candida species, C. auris, in the C. haemulonii complex (Metchnikowiaceae), was first described from a patient in Japan after its isolation from the external ear canal [23]. The species exhibits a close phylogenetic relationship to C. haemulonii and is differentiated based on sequence analysis of the D1/D2 domain of the large ribosomal subunit (LSU) of 26S rRNA gene and the internal transcribed spacer (ITS) regions of the nuclear rRNA gene operon [23]. The first 3 cases of nosocomial fungemia due to C. auris reported in 2011 from South Korea highlighted the fact that this yeast is commonly misidentified as C. haemulonii and Rhodotorula glutinis by the commercial identification systems VITEK (BioMérieux, Marcy l’Etoile, France) and API-20C AUX (BioMérieux), respectively [3]. These systems involve precast panels of assimilation/growth tests using sets of carbon and nitrogen compounds and are still widely used for routine identification of yeasts. A comprehensive study from India investigated C. auris prevalence among 102 clinical isolates previously identified as C. haemulonii or C. famata with the VITEK system and found that 88.2% of the isolates were C. auris, as confirmed by ITS sequencing [9]. It is evident from several studies published recently that C. auris in routine microbiology laboratories remains an unnoticed pathogen, as 90% of the isolates characterized by commercial biochemical identification systems are misidentified primarily because of a lack of the yeast in their databases [3–9, 12, 16–19, 24, 25]. Different biochemical systems are used in microbiology laboratories, and the majority of them listed in Table 1 misidentify C. auris. A recent study on validating the identification of C. auris with 4 biochemical identification platforms found that all C. auris isolates were misidentified as R. glutinis by API-20C AUX, as C. haemulonii (except 1, as C. catenulata) by Phoenix (BD-Diagnostics, Sparks, MD), as C. haemulonii by VITEK, and as C. famata, C. lusitaniae, C. guilliermondii, or C. parapsilosis by MicroScan (Beckman Coulter, Pasadena, CA) [25] (Table 1). However, Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is considered a more rapid and robust diagnostic technique for C. auris identification [9, 10, 13, 16]. Currently, the MALDI-TOF MS approach is commercialized by mainly 2 manufacturers, namely MALDI Biotyper (Bruker-Daltonics, Bremen, Germany) and VITEK MS (BioMérieux). The MALDI Biotyper (Bruker-Daltonics) has a database library that contains spectra of 3 strains of C. auris: 2 from Korea and 1 from Japan. Although both the Bruker-Biotyper and VITEK-MS MALDI-TOF systems lack C. auris entries in the FDA-approved libraries, the research-use-only libraries contain the C. auris database in both MALDI-TOF MS systems [25]. Due to the fact that this yeast is MDR, it is important to identify these species correctly in order to provide optimal patient care. 10.1371/journal.ppat.1006290.t001 Table 1 Worldwide reports of Candida auris along with their misidentification using commercial systems and frequency of antifungal resistance. Country Number of Candida auris isolates Sample (number) Biochemical misidentification (System) Molecular/MALDI-TOF MS identification Number of isolates Year of publication [References] FLU(≥32 μg/ml) ITC(≥1 μg/ml) VRC(≥2 μg/ml) Echinocandins(≥8 μg/ml) AMB(>1 μg/ml) Japan 1 Ear discharge ND ITS, D1D2 ND ND ND ND ND 2009 [23] Korea 15 Ear discharge ND ITS, D1D2 8 8 2 none 9 2009 [2] South Korea 6 Blood C. haemulonii (VITEK), Rhodotorula glutinis (API20C-AUX) ITS, D1D2 4 2 none none 3 2011 [3] India 12 Blood C. haemulonii, C. famata (VITEK); C. sake (API20C-AUX) ITS, D1D2 10 none none none none 2013 [4] 15 Blood (7), CVC tip (3), Excised tissue (3), BAL (1), pus (1) C. haemulonii (VITEK) ITS 15 none 7 none none 2014 [5] 4 Blood (1), urine (1), pericardial fluid (1), BAL (1) C. haemulonii (VITEK); C. sake (API20C-AUX) ITS, D1D2 4 none none none none 2014 [6] 102 Blood (78), tissue (4), pleural fluid (6), peritoneal fluid (7), urine (4), sputum (3) C. haemulonii/C. famata (VITEK) ITS, MALDI-TOF MS 80 none 32 none 14 2015 [9] 51 Blood Not mentioned ITS, D1D2 49 3 9 none 10 2017 [19] India, South Africa, Korea, Japan, Brazil 104: 90 India (I), 6 South Africa (SA), 5 Brazil (B), 2 Korea (K), 1 Japan (J) Blood (n = 89; 78 I, 6 SA, 5 B), peritoneal and pleural fluid (5), invasive infections (4), urine (1), sputum (2) C. haemulonii (VITEK) ITS, D1D2, MALDI-TOF MS 5 (SA); 5 (B); 1 (K); none (J) None (SA); none (B); 1 (K); none (J) 1 (SA); 5 (B); 1 (K); none (J) none (SA); none (B); none (K); none (J) none (SA); 3 (B); none (K); none (J) 2016 [10] a Kuwait 1 Blood C. haemulonii (VITEK) ITS, D1D2 1 ND none none none 2015 [8] Israel 6 Blood (5), urine (1) C. haemulonii (VITEK) ITS, D1D2 6 none none none 6 2017 [18] Spain 8 Blood (4), catheter tip (4) Saccharomyces cerevisiae (AuxaColor 2); C. sake (API20C-AUX); C. lusitaniae, C. haemulonii (VITEK) ITS 8 none 8 none none 2017 [17] UK 12 Blood, sputum, CSF, pleural fluid, arterial line, pustule swab, wound swab, femoral line ITS, D1D2, MALDI-TOF MS 5 ND 1 none none 2016 [11] 50 Blood (16), wound (3), urinary catheter (1), unknown site with invasive candidiasis (2), colonization (28) b MALDI-TOF MS 50 ND ND none Range 0.5–2 μg/ml 2016 [13] Kenya 21 Blood C. haemulonii (VITEK) ITS - - - - - 2014 [24] South Africa 4 Blood C. haemulonii (VITEK) and R. glutinis (API20C-AUX) ITS, D1D2 4 none 1 none none 2014 [7] US 7 Blood (5), urine (1), external ear canal (1) Whole genome sequencing 5 c ND ND 1 c 1 c 2016 [14] CDC Collaborative Project [Pakistan (n = 18), India (n = 19), South Africa, (n = 10), Venezuela (n = 5), Japan (n = 1)] 54 Blood (27), urine (10), soft tissue (5), other sites (12) D1D2, Whole genome sequencing 50 Range 0.125–2 μg/ml 29 4 19 2017 [15] US 10 NA R. glutinis (API20C-AUX); C. haemulonii, C. catenulata (BD Phoenix); C. haemulonii (VITEK); C. famata, C. lusitaniae, C. guilliermondii, or C. parapsilosis (MicroScan) ITS, D1D2, MALDI-TOF MS ND ND ND ND ND 2017 [25] US, tested strains from Germany (n = 2), India (n = 11), Korea (n = 2), Japan (n = 1) 16 Blood (15), ear (1) Unidentified (API20C-AUX) ITS 8 5 5 d none 12 e , 16 d 2017 [20] Venezuela 18 Blood C. haemulonii (VITEK) ITS 18 ND 18 none Range 1–2 μg/ml 2016 [12] Colombia 17 Blood (13) peritoneal fluid (1), CSF (1), bone (1), urine (1) C. haemulonii (VITEK, Phoenix); C. tropicalis (MicroScan Walkaway); C. famata (API Candida); C. albicans (MicroScanautoSCAN); C. tropicalis (MicroScan Walkaway)/ C. famata (API Candida); C. albicans (MicroScanAutoSCAN) MALDI-TOF MS 10 ND 4 none 11 2017 [16] Abbreviations: -, not clear in the abstract; AMB, amphotericin B; BAL, bronchoalveolar lavage; CDC, US Centers for Disease Control and Prevention; CSF, cerebral spinal fluid; CVC tip, central venous catheter tip; FLU, fluconazole; ITC, itraconazole; ITS, internal transcribed spacer; MALDI-TOF MS, Matrix- assisted laser desorption ionization–time of flight mass spectrometry; MIC, minimum inhibitory concentration; ND, not done; VRC, voriconazole. aAntifungal susceptibility testing data of Indian isolates is same as reported by Kathuria et al., 2015. b Colonization with C. auris was defined as culture-positive skin, oropharynx, vascular line exit site, respiratory, and urinary tract without clinical signs of Candida infection. cMIC value not given. dMICs read after 48 hours. eMICs read after 24 hours. Does genetic predisposition make C. auris virulent? A recently published draft genome of C. auris shows that it has a genome size of approximately 12.3 Mb [26, 27]. A significant percentage of genes in C. auris are devoted to central metabolism, a property that is common to pathogenic Candida and crucial for adaptation to divergent environments. In addition, C. auris shares numerous virulence attributes with C. albicans, including genes and pathways involved in cell wall modelling and nutrient acquisition, histidine kinase-2 component systems, iron acquisition, tissue invasion, enzyme secretion, and multidrug efflux [21, 26, 27]. However, in vitro results in a single study that tested the production of phospholipase and secreted proteinase in multiple isolates of C. auris from different geographical regions showed that both secreted proteinase and phospholipase production was strain dependent. The phospholipase activity and secreted proteinase were detected in 37.5% and 64% of the tested isolates, respectively [20]. In general, the tested C. auris strains tended to have weak phospholipase activity, with the majority of isolates being non-phospholipase producers [20]. Furthermore, a significant portion of the C. auris genome encodes the ATP-binding cassette (ABC) and major facilitator superfamily (MFS) transporter families along with drug transporters that may explain the exceptional multidrug resistance in this pathogen [21, 27]. ABC-type efflux activity by Rhodamine 6G transport was significantly greater among C. auris than C. glabrata isolates, suggesting the intrinsic resistance of C. auris to azoles [18]. Interestingly, comparison of whole genome sequencing (WGS) data shows C. auris to be a close phylogenetic relative of C. lusitaniae, a species recognized for intrinsic antifungal resistance [21, 27]. C. auris also demonstrates thermotolerance, growing optimally at 37°C and maintaining viability at up to 42°C, salt tolerance, and cell aggregation into large, difficult-to-disperse clusters, which may help some strains to persist in the hospital environment [11, 23]. In a Galleria mellonella model, the aggregate-forming isolates exhibit significantly less pathogenicity than their non-aggregating counterparts [11]. Importantly, the non-aggregating isolates exhibited pathogenicity comparable to that of C. albicans, which is the most pathogenic member of the genus [11]. However, it is important to mention here that the observations made in this study are yet to be correlated with clinical cases and thus, assuming the same results in patients, need further experimentation. Furthermore, the virulence of C. auris tested in a mouse model of hematogenous-disseminated candidiasis showed distinct yeast cell aggregates in the kidneys of mice, with lethal C. auris infection suggesting that aggregation might be a mode of immune evasion and persistence in tissue [18]. Another significant factor involved in C. auris virulence is its ability to differentially adhere to polymeric surfaces, form biofilms, and resist antifungal agents that are active against its planktonic counterparts [28]. However, a more recent study reported that C. auris biofilms were significantly thinner, i.e., exhibited 50% thickness compared to C. albicans biofilm [20]. Also, C. auris exhibits minimal ability to adhere to silicone elastomer (a representative catheter material) relative to C. albicans [20]. C. auris’s weak adherence ability suggests that it is likely to play some role in catheter-associated candidiasis but not a large one, in contrast to C. albicans and C. parapsilosis, which are known to cause such infections [20]. Although, C. auris expresses several virulence factors, albeit to a lesser extent than C. albicans and in a strain-dependent manner [20]. The past and present of C. auris: Is the emergence of C. auris a menace to public health? In 2009, 15 isolates of C. auris were recovered from the ear canals of patients suffering from chronic otitis media in South Korea [2]. Most of these isolates showed a reduced susceptibility to AMB and azole antifungals. This report was followed by the first 3 cases of nosocomial fungemia caused by C. auris from South Korea [3]. The latter study reported that the earliest isolate of C. auris was found in 1996 in the Korean isolate collection [3]. All 3 patients had persistent fungemia for 10 to 31 days, and 2 patients who received FLU therapy followed by AMB showed therapeutic failure and had fatal outcome. Subsequently, 2 larger series of candidemia and deep-seated infections from India in 2013 and 2014 clearly showed that clonal strains of MDR C. auris had emerged in 3 hospitals [4, 5]. The isolates were resistant to FLU and 5-flucytosine (FC) and had elevated minimum inhibitory concentrations (MICs) of voriconazole (VRC) and caspofungin (CFG) [4, 5]. The most worrisome findings were persistent candidemia and high attributable mortality rates [4, 5]. C. auris accounted for >5% of candidemia in a national ICUs survey and up to 30% of candidemia at individual hospitals in India [4, 19]. In the subsequent 2 years, several reports of hospital-associated infections emerged from South Africa, United Kingdom, Venezuela, Colombia, United States, Pakistan, Israel, Kenya, and Spain [7, 11–18, 24]. Table 1 lists several countries reporting C. auris infection published so far across 5 continents. A collaborative project undertaken by the US Centers for Disease Control and Prevention (CDC) to understand the global emergence and epidemiology of C. auris reported that isolates from 54 patients with C. auris infection from Pakistan, India, South Africa, and Venezuela showed that 93% of isolates were resistant to FLU, 35% to AMB, and 7% to echinocandins; 41% were resistant to 2 antifungal classes, and 4% were resistant to 3 classes [15]. The fact that this yeast exhibits MDR clonal strains that are nosocomially transmitted is unusual in other Candida species [3, 5, 21]. Therefore, the possible threat of rapid spread in affected countries and its emergence in unaffected countries will not only challenge clinicians for its effective therapeutic management but will also bring high economic burden, especially to countries in resource-limited settings where modern identification facilities and access to antifungals other than FLU are limited. What are the drivers of clonal transmission and nosocomial outbreaks of C. auris? There is increasing evidence that suggests likely transmission of C. auris in healthcare settings. Recent reports highlight the persistent colonization by C. auris of hospital environments and multiple body-sites of patients, leading to high transmissibility and protracted outbreaks [13, 14]. A large outbreak of 50 C. auris cases in a London cardio-thoracic center between April 2015 and July 2016 showed persistent presence of the yeast around bed-space areas [13]. Genotyping with amplified fragment length polymorphism (AFLP) demonstrated that C. auris isolates clustered. Similarly, the investigation of the first 7 cases of C. auris infection identified in the US, which occurred between May 2013 and August 2016, showed colonization with C. auris on skin and other body sites weeks to months after their initial infection, which could possibly lead to contamination of the healthcare environment and pose a risk of continuous transmission [14]. Furthermore, C. auris was isolated from samples taken from the mattress, bedside table, bed rail, chair, and windowsill [14]. WGS results demonstrate that isolates from patients admitted to the same hospital in New Jersey were nearly identical, as were isolates from patients admitted to the same Illinois hospital [14]. Also, in the London outbreak, a healthcare worker caring for a heavily C. auris–colonized patient had a C. auris–positive nose swab [13]. Effective implementation of strict infection-prevention control measures are required to prevent transmission of C. auris. These include isolation of patients and their contacts, wearing of personal protective clothing by healthcare workers, screening of patients on affected wards, skin decontamination with chlorhexidine, environmental cleaning with chlorine-based reagents, and terminal decontamination with hydrogen peroxide vapor or ultraviolet (UV) light [13, 29]. Enhanced terminal cleaning with UV light has recently been shown to reduce infections with many nosocomial pathogens and might also be of use for preventing C. auris transmission [30]. Previously, several geographically related clusters have been reported from South Korea [2, 3], India [4, 5, 10], South Africa [10], Pakistan [15], and hospitals in Latin America [12, 16]. Clonality within C. auris has been shown using AFLP, multilocus sequence typing, and MALDI-TOF MS among strains in India, South Africa, and Brazil [10]. A recent study applying WGS demonstrated highly related C. auris isolates in 4 unrelated and geographically separated Indian hospitals, suggesting that this pathogen exhibits a low diversity [21]. A large-scale application of WGS analysis suggests recent independent and nearly simultaneous emergence of different clonal populations on 3 continents, demonstrating highly related C. auris isolates in the same geographic areas [15]. So far, no reservoir of C. auris has been identified, although future studies on its isolation from animals, plants, and water sources are warranted. Is antifungal resistance in C. auris a therapeutic challenge? Patients with C. auris infections have risk factors similar to those of other Candida spp. infections, including abdominal surgery (25%–77%), broad-spectrum antibiotics (25%–100%), ICU admission (58%), diabetes mellitus (18%), presence of central venous catheters (25%–94%), and malignancies (11%–43%) [3–5, 7, 12, 14–16]. The overall crude in-hospital mortality rate of C. auris candidemia ranges from 30% to 60%, and infections typically occur several weeks (10‒50 days) after admission [4, 5, 10, 12, 13]. C. auris invasive infections represent a therapeutic challenge, and no consensus exists for optimal treatment. A few studies report breakthrough fungemia while on FLU, and this correlates with commonly reported high MICs (>32 μg/ml), suggesting intrinsic resistance against this drug [3–5]. Although epidemiological cutoff values (ECVs) or clinical breakpoints are not yet defined for C. auris, newer azoles such as posaconazole (range, 0.06–1 μg/ml) and isavuconazole (range, 2 μg/ml) MICs [9, 15]. Up till now, echinocandin resistance is noted in fewer isolates (2%–8%) [9, 14, 15], but almost half of isolates are MDR (resistant to ≥2 antifungal classes), and a low number (4%) exhibit resistance to all classes of antifungals [2, 9, 12, 15, 16, 19]. Echinocandins remain the first-line therapy for C. auris infections, provided that specific susceptibility testing is undertaken at the earliest opportunity. Although CFG is normally highly effective against Candida biofilms, a recent report demonstrated that CFG was predominately inactive against C. auris biofilms [29]. FC (MIC50, 0.125–1 μg/ml) is a treatment option in renal tract or urinary tract infections, as the echinocandins fail to achieve therapeutic concentrations in urine [4, 5, 7, 9, 11–13, 15, 18]. Also, a novel drug, SCY-078, which is the first orally bioavailable 1, 3-β-D-glucan synthesis inhibitor, has been shown to possess potent activity against various Candida spp. and exhibit potent antifungal activity against C. auris isolates [20]. Furthermore, SCY-078 showed growth-inhibition and anti-biofilm activity and could be an important antifungal to treat this MDR species [20]. At present, the mechanism of antifungal resistance in C. auris is unclear. The recently published draft genome of C. auris revealed the presence of single copies of ERG3, ERG11, FKS1, FKS2, and FKS3 genes [21]. Detection of azole-resistant mutations by comparing ERG11 amino acid sequences between C. albicans and C. auris showed that alterations at azole-resistance codons in C. albicans were present in C. auris isolates [15]. These substitutions were strongly associated with country-wise–specific geographic clades [15]. Resistance is probably inducible under antifungal pressure, resulting in rapid mutational changes. However, future studies with emphasis on several molecular mechanisms, including efflux and transporters, could provide insight on C. auris resistance. What are the important things that we still need to learn about C. auris? We are just beginning to know the epidemiology and behavior of C. auris, but at the present, far more gaps exist in our knowledge. The earliest findings of C. auris are from 1996. The pertinent question remains whether this pathogen existed far earlier than 1996, and we were just unable to identify it. The latter is less plausible because many centers have reviewed archived isolate collections that have not shown any isolates of C. auris before 1996. We also do not know why C. auris is independently, almost simultaneously, emerging in so many places worldwide. It has been shown that there is a profound phylo-geographic structure with large genetic differences among geographic clades and high clonality within the geographic clades. However, a common characteristic is the high level of antifungal resistance, which is rare in other Candida spp. C. auris is the only species in which several isolates have been identified with resistance to all 4 classes of human antifungal drugs. It seems reasonable to opine that changes or misuse of antifungal drugs is one of the factors, although no specific risk factors for acquiring C. auris seem to exist. What we do know is that environmental factors probably play a role in outbreaks in healthcare settings that include prolonged survival in healthcare environments, probably due to skin colonization of patients and asymptomatic carriers. It is obvious that future research is warranted on multiple aspects of C. auris, which seems to have the typical characteristics of well-known, healthcare-associated pathogens such as carbapenemase-producing gram-negatives, Clostridium difficile, vancomycin-resistant Enterococcus (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Given the behavior of the latter 4, a further spread of C. auris in healthcare settings on a worldwide scale is expected. C. auris worldwide emergence has prompted the CDC, (http://www.cdc.gov/fungal/diseases/candidiasis/candida-auris-alert.html [last accessed February 2017]), Public Health England (PHE), London (https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/534174/Guidance_Candida__auris.pdf [last accessed February 2017]), and the European Centre for Disease Prevention and Control (ECDC), Europe (http://ecdc.europa.eu/en/publications/Publications/Candida-in-healthcare-settings_19-Dec-2016.pdf) to issue health alerts for strict vigilance of C. auris cases. International collaborative consortia and timely efforts by the medical community are indispensable in controlling this super bug before it adapts in our healthcare facilities. Furthermore, more intensive efforts are required, and one such crucial step is the support from funding agencies to initiate multidisciplinary research to better understand its ecology, evolution, and resistance mechanisms, which will go a long way for its treatment and prevention.
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              Attributable mortality of nosocomial candidemia, revisited.

              We reexamined the attributable mortality of nosocomial candidemia 15 years after a retrospective cohort study performed at our hospital demonstrated an attributable mortality of 38%. For all episodes of nosocomial candidemia between 1 July 1997 and 30 June 2001, we matched control patients with case patients by age, sex, date of hospital admission, underlying disease(s), length of time at risk, and surgical procedure(s). We analyzed 108 matched pairs. There were no statistically significant differences in age, sex, underlying disease(s), time at risk, surgical procedure, or vital signs at admission between cases and controls. The crude mortality among case patients was 61% (66 of 108 patients), compared with 12% (13 of 108) among control patients, for an attributable mortality of 49% (95% CI, 38%-60%). Nosocomial candidemia is still associated with an extremely high crude and attributable mortality--much higher than that expected from underlying disease alone.
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                Author and article information

                Journal
                J Fungi (Basel)
                J Fungi (Basel)
                jof
                Journal of Fungi
                MDPI
                2309-608X
                01 October 2019
                December 2019
                : 5
                : 4
                : 92
                Affiliations
                Department of Biology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA; gina.wall@ 123456utsa.edu (G.W.); n-herrera@ 123456live.com (N.H.)
                Author notes
                [* ]Correspondence: jose.lopezribot@ 123456utsa.edu ; Tel.: +1-210-458-7022
                Author information
                https://orcid.org/0000-0003-1992-3270
                Article
                jof-05-00092
                10.3390/jof5040092
                6958377
                31581540
                aa9a22bb-b955-47b3-b86e-f43f1cc69b66
                © 2019 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 16 September 2019
                : 29 September 2019
                Categories
                Article

                candida auris,antifungals,drug screening,pathogen box,repurposing

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