71
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Identification and screening of cardiac glycosides in Streptocaulon griffithii using an integrated data mining strategy based on high resolution mass spectrometry

      research-article

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The present study was designed to develop a practical strategy to tackle the problem of lacking standard compounds and limited references for identifying structure-related compounds in Streptocaulon griffithii Hook. f., especially those in trace concentrations, with a focus on antitumor activity. The cardiac glycosides (CGs)-enriched part was determined using in vitro bioactive assays in three cancer cell lines and then isolated using macroporous resins. The MS and MS/MS data were acquired using a high performance liquid chromatography coupled with hybrid quadrupole-time of flight (HPLC-Q-TOF-MS) system. To acquire data of trace compound in the extract, a multiple segment program was applied to modify the HPLC-Q-TOF-MS method. A mass defect filter (MDF) approach was employed to make a primary MS data filtration. Utilizing a MATLAB program, the redundant peaks obtained by imprecise MDF template calculated with limited references were excluded by fragment ion classification, which was based on the ion occurrence number in the MDF-filtered total ion chromatograms (TIC). Additionally, the complete cleavage pathways of CG aglycones were proposed to assist the structural identification of 29 common fragment ions (CFIs, ion occurrence number ≥ 5) and diagnostic fragment ions (DFIs, ion occurrence number < 5). As a result, 30 CGs were filtered out from the MDF results, among which 23 were identified. This newly developed strategy may provide a rapid and effective tool for identifying structure-related compounds in herbal medicines.

          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 July 2018
          : 16
          : 7
          : 546-560
          Affiliations
          [1] 1Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing 210009, China
          [2] 2Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing 211198, China
          Author notes
          *Corresponding author: LIU Wen-Yuan, E-Mail: liuwenyuan8506@ 123456163.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(18)30090-6
          10.1016/S1875-5364(18)30090-6
          aac142af-19a1-4df2-bc55-6e8390d9546f
          Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          History
          : 17 April 2018
          Funding
          Funded by: National Natural Science Foundation of China
          Award ID: 81673567
          Award ID: 81703382
          This work was supported by the National Natural Science Foundation of China (Nos. 81673567 and 81703382).

          Medicine,Pharmaceutical chemistry,Pharmacology & Pharmaceutical medicine,Complementary & Alternative medicine
          Streptocaulongriffithii ,Cardiac glycosides,Mass defect filter,HPLC-Q-TOF-MS

          Comments

          Comment on this article