15
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Evaluation of an immunochromatography rapid diagnosis kit for detection of chikungunya virus antigen in India, a dengue-endemic country

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Chikungunya virus (CHIKV) and dengue virus (DENV) are arboviruses that share the same Aedes mosquito vector, and there is much overlap in endemic areas. In India, co-infection with both viruses is often reported. Clinical manifestations of Chikungunya fever is often confused with dengue fever because clinical symptoms of both infections are similar. It is, therefore, difficult to differentiate from those of other febrile illnesses, especially dengue fever. We previously developed a CHIKV antigen detection immunochromatography (IC) rapid diagnosis kit [ 1]. The current study examined the efficacy of previously mentioned IC kit in India, a dengue-endemic country.

          Methods

          Sera from 104 CHIKV-positive (by qRT-PCR) and/or IgM-positive (ELISA) subjects collected in 2016, were examined. Fifteen samples from individuals with CHIKV-negative/DENV-positive and 4 samples from healthy individuals were also examined. Of the 104 CHIKV-positive sera, 20 were co-infected with DENV.

          Results

          The sensitivity, specificity and overall agreement of the IC assay were 93.7, 95.5 and 94.3%, respectively, using qRT-PCR as a gold standard. Also, there was a strong, statistically significant positive correlation between the IC kit device score and the CHIKV RNA copy number. The IC kit detected CHIKV antigen even in DENV-co-infected patient sera and did not cross-react with DENV NS1-positive/CHIKV-negative samples.

          Conclusions

          The results suggest that the IC kit is useful for rapid diagnosis of CHIKV in endemic areas in which both CHIKV and DENV are circulating.

          Electronic supplementary material

          The online version of this article (10.1186/s12985-018-1000-0) contains supplementary material, which is available to authorized users.

          Related collections

          Most cited references8

          • Record: found
          • Abstract: found
          • Article: not found

          Early diagnosis of Dengue infection using a commercial Dengue Duo rapid test kit for the detection of NS1, IGM, and IGG.

          A commercial Dengue Duo rapid test kit was evaluated for early dengue diagnosis by detection of dengue virus NS1 antigen and immunoglobulin M (IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-house IgM capture enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition assay, and the SD Dengue Duo rapid test. Of the 320 dengue acute and convalescent sera, dengue infection was detected by either serology or RT-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies. The sensitivity and specificity in diagnosing acute dengue infection in the SD Duo NS1/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific. Detection of both NS1 and IgM by SD Duo gave comparable detection rate by either serology or RT-PCR.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Genetic characterization of Chikungunya virus from New Delhi reveal emergence of a new molecular signature in Indian isolates

            Background Chikungunya (CHIK) is currently endemic in South and Central India and exist as co-infections with dengue in Northern India. In 2010, New Delhi witnessed an outbreak of CHIK in the months October-December. This was the first incidence of a dominant CHIK outbreak in Delhi and prompted us to characterize the Delhi virus strains. We have also investigated the evolution of CHIK spread in India. Findings Clinical samples were subjected to RT-PCR to detect CHIK viral RNA. The PCR amplified products were sequenced and the resulting sequences were genetically analyzed. Phylogenetic analysis based on partial sequences of the structural proteins E1 and E2 revealed that the viruses in the latest outbreak exhibited ECSA lineage. Two novel mutations, E1 K211E and E2 V264A were observed in all Delhi isolates. In addition, CHIKV sequences from eight states in India were analyzed along with Delhi sequences to map the genetic diversity of CHIKV within the country. Estimates of average evolutionary divergence within states showed varying divergence among the sequences both within the states and between the states. We identified distinct molecular signatures of the different genotypes of CHIKV revealing emergence of a new signature in the New Delhi clade. Statistical analyses and construction of evolutionary path of the virus within the country revealed gradual spread of one specific strain all over the country. Conclusion This study has identified unique mutations in the E1 and E2 genes and has revealed the presence of ancestral CHIKV population with maximum diversity circulating in Maharashtra. The study has further revealed the trend of CHIK spread in India since its first report in 1963 and its subsequent reappearance in 2005.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              A Re-Examination of the History of Etiologic Confusion between Dengue and Chikungunya

              Goro Kuno (2015)
              Contrary to the perception of many researchers that the recent invasion of chikungunya (CHIK) in the Western Hemisphere marked the first episode in history, a recent publication reminded them that CHIK had prevailed in the West Indies and southern regions of the United States from 1827–1828 under the guise of “dengue” (DEN), and that many old outbreaks of so-called “dengue” actually represented the CHIK cases erroneously identified as “dengue.” In hindsight, this confusion was unavoidable, given that the syndromes of the two diseases—transmitted by the same mosquito vector in urban areas—are very similar, and that specific laboratory-based diagnostic techniques for these diseases did not exist prior to 1940. While past reviewers reclassified problematic “dengue” outbreaks as CHIK, primarily based on manifestation of arthralgia as a marker of CHIK, they neither identified the root cause of the alleged misdiagnosis nor did they elaborate on the negative consequences derived from it. This article presents a reconstructed history of the genesis of the clinical definition of dengue by emphasizing problems with the definition, subsequent confusion with CHIK, and the ways in which physicians dealt with the variation in dengue-like (“dengue”) syndromes. Then, the article identifies in those records several factors complicating reclassification, based on current practice and standards. These factors include terms used for characterizing joint problems, style of documenting outbreak data, frequency of manifestation of arthralgia, possible involvement of more than one agent, and occurrence of the principal vector. The analysis of those factors reveals that while some of the old “dengue” outbreaks, including the 1827–1828 outbreaks in the Americas, are compatible with CHIK, similar reclassification of other “dengue” outbreaks to CHIK is difficult because of a combination of the absence of pathognomonic syndrome in these diseases and conflicting background information.
                Bookmark

                Author and article information

                Contributors
                jaspreet.jain@gmail.com
                okbys81@cc.miyazaki-u.ac.jp
                navjotmarwah@gmail.com
                emien@biken.osaka-u.ac.jp
                shioda@biken.osaka-u.ac.jp
                +91-11-26198412 , rgaind5@hotmail.com
                +81 42 561 0771 , kurosu@niid.go.jp
                +91-11-26741358 , sujatha@icgeb.res.in
                Journal
                Virol J
                Virol. J
                Virology Journal
                BioMed Central (London )
                1743-422X
                11 May 2018
                11 May 2018
                2018
                : 15
                : 84
                Affiliations
                [1 ]ISNI 0000 0004 0498 7682, GRID grid.425195.e, Vector Borne Disease Group, , International Centre for Genetic Engineering and Biotechnology, ; New Delhi, India
                [2 ]ISNI 0000 0001 0657 3887, GRID grid.410849.0, Department of Veterinary science, Faculty of Agriculture, , University of Miyazaki, ; Musashimurayama, Japan
                [3 ]ISNI 0000 0001 0657 3887, GRID grid.410849.0, Center for Animal Disease Control, , University of Miyazaki, ; Musashimurayama, Japan
                [4 ]ISNI 0000 0004 0373 3971, GRID grid.136593.b, Mahidol Osaka Center for Infectious Diseases, , Osaka University, ; Musashimurayama, Japan
                [5 ]ISNI 0000 0004 1803 7549, GRID grid.416888.b, Department of Microbiology, , Vardhman Mahavir Medical College & Safdarjung Hospital, ; New Delhi, India
                [6 ]ISNI 0000 0004 0373 3971, GRID grid.136593.b, Research Institute of Microbial Diseases, Osaka University, ; Osaka, Japan
                [7 ]ISNI 0000 0001 2220 1880, GRID grid.410795.e, Special Pathogens Laboratory, Department of Virology 1, , National Institute of Infectious Diseases, ; 4-7-1 Gakuen Musashimurayama, Musashimurayama, Japan
                Article
                1000
                10.1186/s12985-018-1000-0
                5948817
                29751761
                aadf5f75-d932-4997-80e7-cd9050802088
                © The Author(s). 2018

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 8 February 2018
                : 7 May 2018
                Funding
                Funded by: Department of Science and Technology, Government of India
                Funded by: Japan Initiative for Global Research Network on Infectious Diseases, AMED
                Categories
                Research
                Custom metadata
                © The Author(s) 2018

                Microbiology & Virology
                chikungunya virus,early diagnosis,dengue co-infection,immunochromatography,mosquito-borne disease

                Comments

                Comment on this article