Objective To investigate the changes of the contents of different immune cells and the expression of cell surface molecules in the spleen of C57BL/6 mice infected with Plasmodium berghei ANKA.
Methods The number of 10 C57BL/6 mice were divided into the normal group and the infected group with 5 in each group, and normal saline and Plasmodium berghei were injected into the tail vein separately. Six days later, the spleens of all mice were isolated to prepare a single cell suspension. The percentage of macrophages and DCs and their cell surface molecules CD69, MHC Ⅱ , CD80 and TLR2 from the mice were detected by flow cytometry.
Results The proportion of spleen macrophages in the infected group was lower than that in the normal group without significant difference ( P>0.05), the expression of the surface factor CD69 and CD80 of spleen macrophages in the infected group were significantly higher than those in the normal group ( P<0.01), the surface molecule MHC Ⅱ of it was also higher than that of the normal group, but there was no significance ( P>0.05), and the percentage of TLR2 was lower than that of the normal group, and there was no significance ( P>0.05). The proportion of DC in the infected group was statistically lower than that in the normal group ( P<0.05), the expression of the CD69 and CD80 of spleen DC cells in the infected group were significantly higher than those in the normal group ( P<0.01), the proportion of surface molecule TLR2 and MHC Ⅱ was lower than those in the normal group, but the differences were not statistically significant ( P>0.05).
Conclusion The content of DC in the spleen of C57BL/6 mice infected with Plasmodium berghei decreased, and the proportion of the cytokines CD69 and CD80 expressed by macrophages and DCs increased. The authors believe that macrophages and DCs mainly resist the infection of Plasmodium berghei by increasing the expression of CD69 and CD80.
摘要： 目的 探讨 C57BL/6 小鼠感染伯氏疟原虫 ANKA 株后脾脏不同免疫细胞的含量及其表达的细胞表面分子的 变化。 方法 将 10 只 C57BL/6 小鼠分为正常对照组和感染组, 每组 5 只, 分别经尾静脉注射生理盐水和伯氏疟原虫虫 株, 6 天后分离小鼠脾脏, 制备单细胞悬液。通过流式细胞术检测小鼠巨噬细胞 (Mφ) 和树突状细胞 (DC) 及其细胞表面 分子 CD69、MHCⅡ、CD80、TLR2 的百分比含量。 结果 感染组小鼠脾脏巨噬细胞所占比例低于正常组, 差异无统计学 意义 ( P>0.05); 其表面分子 CD69、CD80、MHCⅡ的表达水平较正常组均升高, 且 CD69、CD80 的表达差异有统计学意义 ( P<0.01) , 而 MHCⅡ的表达差异无统计学意义 ( P>0.05); 其表达的 TLR2 百分比较正常组降低, 差异无统计学意义 ( P> 0.05) 。感染组小鼠 DC 所占比例低于正常组, 差异有统计学意义 ( P<0.05); 感染组小鼠脾脏 DC 表达 CD69、CD80 水平 较正常组均显著升高, 差异有统计学意义 ( P<0.01); 其表达 TLR2、MHCⅡ所占比例均低于正常组, 差异无统计学意义 ( P>0.05) 。 结论 感染伯氏疟原虫的 C57BL/6 小鼠脾脏中 DC 含量降低, 巨噬细胞和 DC 表达细胞因子 CD69、CD80 的 能力上升, 由此笔者认为巨噬细胞和 DC 可能会通过增加其自身 CD69 和 CD80 的表达水平来抵抗伯氏疟原虫的感染。