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Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro Translated title: Tratamento de plantas matrizes de Chrysanthemum morifolium cv. "Bi Time" com Ca2+, K+ E Mg2+ e calogênese in vitro

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      Abstract

      The chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight was affected by nutrients treatment. Callus growth on leaf explants was inversely proporcional to potassium concentration and directly proportional to magnesium concentration in shoot explants. The calcium effect on callogensis of leaf explants was dependent on treatment duration. For 30 days treatment callogenseis was inversely related to calcium concentration and after 45 days was directly related to calcium concentration.

      Translated abstract

      A composição química e o vigor vegetativo de plantas matrizes são essenciais no desenvolvimento de explantes in vitro. Com o objetivo de testar o efeito do estado nutricional de plantas matrizes de Chrysanthemum morifolium no processo de calogênese in vitro, plantas crescendo em vasos contendo areia lavada foram irrigadas com soluções nutritivas contendo diferentes concentrações de cálcio (0, 50, 100 e 200 mg L-1), potássio (0, 58,5, 117 e 234 mg L-1), e magnésio (0,12, 48 e 96 mg L-1). Aos 30 e 45 dias de duração do tratamento com solução nutritiva, explantes (segmento caulinar e foliar) das matrizes foram coletados, desinfestados e inoculados em meio de cultura MS sólido suplementado com 0,1 mg L-1 de Kin e 5,0 mg L-1 NAA. A calogênese foi afetada pelos tratamentos com os diferentes níveis dos nutrientes. O crescimento de calos a partir de explantes foliares foi inversamente proporcional ao teor de potássio e diretamente proporcional aos de magnésio em explantes caulinares. O efeito do cálcio na calogênese em explantes foliares mostrou ser dependente da duração do tratamento. Nos testes com 30 dias de duração a calogênese foi inversamente proporcional, e naqueles com 45 dias de duração demonstrou ser diretamente proporcional aos níveis de cálcio.

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        Beneficial Mineral Elements

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          Cycling of Amino-Nitrogen and other Nutrients between Shoots and Roots in Cereals—A Possible Mechanism Integrating Shoot and Root in the Regulation of Nutrient Uptake

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            Author and article information

            Affiliations
            [1 ] Universidade de São Paulo Brazil
            [2 ] Universidade de São Paulo Brazil
            Contributors
            Role: ND
            Role: ND
            Role: ND
            Role: ND
            Journal
            sa
            Scientia Agricola
            Sci. agric. (Piracicaba, Braz.)
            São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" (Piracicaba )
            1678-992X
            December 2002
            : 59
            : 4
            : 689-693
            S0103-90162002000400011
            10.1590/S0103-90162002000400011

            http://creativecommons.org/licenses/by/4.0/

            Product
            Product Information: SciELO Brazil
            Categories
            AGRICULTURE, MULTIDISCIPLINARY

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