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      Normalization of the Measurement of Cardiac Creatine Phosphokinase Activity

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          Abstract

          Cardiac creatine phosphokinase (CPK) activity in international units (IU) was normalized to homogenate soluble protein (IU/mg), to tissue wet weight (IU/100 mg) or to tissue dry weight (IU/mg). Samples of cardiac tissue were excised from sham-operated cats and dogs or from cats after 4 h of severe pericardial tamponade and dogs after 4 h of circumflex artery ligation. In sham-operated cats and dogs, cardiac CPK activity was about 30 IU/mg homogenate soluble protein, 200 IU/100 mg wet tissue, and 9.5 IU/mg dry tissue. In cats, after tamponade, the CPK activity of subepicardial and subendocardial tissue of the left ventricle was decreased 24–35% if the enzyme activity was referenced to wet weight or dry weight. Similarly, in dogs, after circumflex artery ligation, the CPK activity of posterior papillary muscles was reduced 24–30% if the enzyme activity was referenced to tissue wet or dry weight. In both cats subjected to 4 h of tamponade and dogs after 4 h of circumflex artery ligation, significant loss of CPK activity could not be demonstrated if the cardiac enzyme activity was normalized only to the soluble protein content of homogenates. We conclude that normalizing cardiac CPK activity to tissue wet and dry weight may permit early assessment of myocardial injury in experimental models of myocardial ischemia.

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          Author and article information

          Journal
          CRD
          Cardiology
          10.1159/issn.0008-6312
          Cardiology
          S. Karger AG
          0008-6312
          1421-9751
          1979
          1979
          31 October 2008
          : 64
          : 4
          : 222-230
          Affiliations
          Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa.
          Article
          170619 Cardiology 1979;64:222–230
          10.1159/000170619
          476729
          ab6b71f2-2fe5-4799-aa82-b051fc06f9b9
          © 1979 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Page count
          Pages: 9
          Categories
          Original Paper

          General medicine,Neurology,Cardiovascular Medicine,Internal medicine,Nephrology
          Myocardial CPK,Creatine phosphokinase,Myocardial ischemia,Cardiac water,Pericardial tamponade

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