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      Use of selective media for detection of cephalothin-resistant bacteria in surgical patients

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          Abstract

          Bacterial colonization in hospitalized patients is an important step in nosocomial infections. Frequent employment of antimicrobials can modify patients' normal microbiota, favoring colonization and infection by antimicrobial-resistant microorganisms. First-generation cephalosporins are frequently used as prophylactic antibiotics in surgery. Intestinal, oropharyngeal and skin colonization by cephalothin-resistant microorganisms were studied in 60 pre-operative patients at the Hospital Universitário Pedro Ernesto. Feces were cultured in Eosin-methylene blue medium containing 32 µg/mL of cephalothin. Swabs obtained from the oropharynx and from skin were inoculated in cistein-lactose electrolytes-deficient medium containing 32 µg/mL of cephalothin. Isolated strains were identified and tested for susceptibility to antimicrobials by disk diffusion. Cephalothin-resistant strains were isolated from the feces of 59 patients (98%), from the oropharynx of 13 patients (22%) and from skin in 10 patients (17%). Enterobacter cloacae was predominant in feces (68% of the patients) and oropharynx (13%). Acinetobacter spp. was the most frequent microorganism isolated from the skin (10%). Antimicrobial multiresistant strains were isolated from at least one of the sites in 38 patients (63%). The employment of selective medium containing antimicrobials is a relatively simple and efficient method, being useful to evaluate microorganisms from hospitalized patients' microbiota that are relevant as potential pathogens in nosocomial infections.

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          Most cited references41

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          Nosocomial Respiratory Infections with Gram-Negative Bacilli

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            Origin of Infection in Acute Nonlymphocytic Leukemia

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              Surgical wound infection

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjid
                Brazilian Journal of Infectious Diseases
                Braz J Infect Dis
                Brazilian Society of Infectious Diseases (Salvador )
                1678-4391
                June 2004
                : 8
                : 3
                : 190-196
                Affiliations
                [1 ] Universidade do Estado do Rio de Janeiro Brazil
                [2 ] Universidade do Estado do Rio de Janeiro Brazil
                [3 ] Universidade do Estado do Rio de Janeiro Brazil
                Article
                S1413-86702004000300001
                10.1590/S1413-86702004000300001
                ac0d2d34-0c76-44c7-88ea-c672ba2054f8

                http://creativecommons.org/licenses/by/4.0/

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                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1413-8670&lng=en
                Categories
                INFECTIOUS DISEASES

                Infectious disease & Microbiology
                Selective media,cephalotin resistance,surgical patients
                Infectious disease & Microbiology
                Selective media, cephalotin resistance, surgical patients

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