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      Digoxin-Like Immunoreacting Substance(s) in the Serum of Patients with Chronic Uremia

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          Abstract

          In patients with chronic uremia we have previously demonstrated a significant inhibition of the Na-K-ATPase enzyme which represents the specific receptor protein for cardiac glycosides. Since an endogenous inhibitor of this enzyme was previously shown to react with a digoxin antibody, in the present study we determined digoxin-like immunoreacting activity(ies) (DLIA) by a radioimmunoassay in 15 nondialyzed patients with chronic renal failure. In native serum, DLIA ranged from 0 to 1.70 ng/ml and was unrelated to the degree of renal failure. After gel filtration of serum, DLIA exclusively eluted in the small molecular weight salt (F III) and post-salt (FIV) fractions and averaged 0.22 ± 0.04 and 0.20 ± 0.05 ng/ml in fractions III and IV, respectively. Total activities ranged from 0.11 to 0.88 ng/ml with a mean of 0.42 ± 0.06 ng/ml and closely correlated with the degree of renal impairment (p < 0.001). The results confirm the presence of small molecular weight digoxin-like immunoreacting substance(s) in uremic serum. The variable activities in native serum and the lack of correlation between the degree of renal failure and DLIA in serum fraction IV previously shown to possess the Na-K-ATPase-inhibiting activity, however, indicate that DLIA may not reflect specifically the endogenous sodium pump inhibitor and that unspecific binding to this digoxin antibody of uremic toxins or other endogenous compounds, such as steroids other than aldosterone, may have occurred.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1985
          1985
          04 December 2008
          : 40
          : 3
          : 297-302
          Affiliations
          Division of Nephrology, Medizinische Poliklinik, University of Bonn, FRG
          Article
          183482 Nephron 1985;40:297–302
          10.1159/000183482
          4010843
          © 1985 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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