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      Stimulation of human red blood cells leads to Ca2+-mediated intercellular adhesion

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          Abstract

          Red blood cells (RBCs) are a major component of blood clots, which form physiologically as a response to injury or pathologically in thrombosis. The active participation of RBCs in thrombus solidification has been previously proposed but not yet experimentally proven. Holographic optical tweezers and single-cell force spectroscopy were used to study potential cell-cell adhesion between RBCs. Irreversible intercellular adhesion of RBCs could be induced by stimulation with lysophosphatidic acid (LPA), a compound known to be released by activated platelets. We identified Ca2+ as an essential player in the signaling cascade by directly inducing Ca2+ influx using A23187. Elevation of the internal Ca2+ concentration leads to an intercellular adhesion of RBCs similar to that induced by LPA stimulation. Using single-cell force spectroscopy, the adhesion of the RBCs was identified to be approximately 100 pN, a value large enough to be of significance inside a blood clot or in pathological situations like the vasco-occlusive crisis in sickle cell disease patients.

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          Author and article information

          Journal
          2011-05-11
          Article
          1105.2314
          d202460f-4459-4160-bfd1-2a40d0fb8615

          http://arxiv.org/licenses/nonexclusive-distrib/1.0/

          History
          Custom metadata
          Manuscript with figures included, 1 supplementary figure, 1 video
          physics.bio-ph q-bio.TO
          B

          Biophysics,Life sciences
          Biophysics, Life sciences

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