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      Fast gas chromatography characterisation of purified semiochemicals from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae).

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          Abstract

          The chemical composition of Matricaria chamomilla L. and Nepeta cataria L. essential oils was determined by GC-MS on an apolar stationary phase by comparison of the characteristic fragmentation patterns with those of the Wiley 275L database. The GC-MS chromatograms were compared with those obtained by fast GC equipped with a direct resistively heated column (Ultra Fast Module 5% phenyl, 5 mx 0.1 mm, 0.1 microm film thickness). Analytical conditions were optimised to reach a good peak resolution (split ratio=1:100), with analysis time lower than 5 min versus 35-45 min required by conventional GC-MS. The fast chromatographic method was completely validated for the analysis of mono- and sesquiterpene compounds. Essential oils were then fractionated by column chromatography packed with silica gel. Three main fractions with high degree of purity in E-beta-farnesene were isolated from the oil of M. chamomilla. One fraction enriched in (Z,E)-nepetalactone and one enriched in beta-caryophyllene were obtained from the oil of N. cataria. These semiochemical compounds could act as attractants of aphid's predators and parasitoids.

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          Author and article information

          Journal
          J Chromatogr A
          Journal of chromatography. A
          1873-3778
          0021-9673
          Apr 3 2009
          : 1216
          : 14
          Affiliations
          [1 ] Department of Analytical Chemistry, Gembloux Agricultural University, Passage des Déportés 2, B-5030 Gembloux, Belgium.
          Article
          S0021-9673(08)01723-8
          10.1016/j.chroma.2008.09.109
          18952218
          ac47773c-cc50-408f-9086-958cb3e85411
          History

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