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      Hemodialysis-lnduced Upregulation of Granulocyte Adhesion Molecules CD 11b/CD18 Is Independent from 5-Lipoxygenase Activity


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          Hemodialysis with new cuprophane membranes upregulates expression of granulocyte adhesion molecules and activates 5-lipoxygenase as reflected by the enhanced generation of leukotriene B<sub>4</sub> (LTB<sub>4</sub>). We assessed the role of 5-lipoxygenase activity in hemodialysis-induced upregulation of the granulocyte adhesion molecules, CD lib and CD 18, and granulocyte adhesion to human umbilical vein endothelial cells in an ex vivo dialysis model. 5-Lipoxygenase was effectively inhibited by preincubation of human whole blood with the speciñc inhibitor, BAY XI00 5. Dialysis with new cuprophane but not with new acrylonitrile membranes significantly upregulated expression of CD1 lb and CD 18 by 6-fold and 4-fold, respectively. Inhibition of 5-lipoxygenase did not affect the expression of CD1 lb and CD 18 during dialysis with either of the two membranes. In contrast to the enhanced expression of CD1 lb and CD 18, adhesion of granulocytes to human umbilical vein endothelial cells did not increase during dialysis, nor was it affected by BAY XI005. These data indicate that ex vivo dialysis with cuprophane membranes upregulates expression of CD1 lb and CD 18 on granulocytes independent of the activation of 5-lipoxygenase.

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          Author and article information

          S. Karger AG
          23 December 2008
          : 76
          : 4
          : 418-424
          aInstitut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten und bMedizinische Klinik, Klinikum Innenstadt, Universität München, Deutschland
          190224 Nephron 1997;76:418–424
          © 1997 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 24 January 1997
          Page count
          Pages: 7
          Original Paper

          Cardiovascular Medicine,Nephrology
          5-Lipoxygenase,Cuprohane,CD lib/CD 18,Hemodialysis
          Cardiovascular Medicine, Nephrology
          5-Lipoxygenase, Cuprohane, CD lib/CD 18, Hemodialysis


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