Zheng Sun 1 , Russell A. Miller 1 , Rajesh T. Patel 1 , Jie Chen 2 , Ravindra Dhir 1 , Hong Wang 3 , Dongyan Zhang 4 , Mark J. Graham 5 , Terry G. Unterman 6 , Gerald I. Shulman 4 , Carole Sztalryd 3 , Michael J. Bennett 2 , 7 , Rexford S. Ahima 1 , Morris J. Birnbaum 1 , Mitchell A. Lazar 1
01 December 2012
Fatty liver disease is associated with obesity and type 2 diabetes, and hepatic lipid accumulation may contribute to insulin resistance by a variety of mechanisms. Here we show that mice with liver-specific deletion of histone deacetylase 3 (Hdac3) display severe hepatosteatosis and, notably increased insulin sensitivity without changes in insulin signaling or body weight. Hdac3 deletion reroutes metabolic precursors towards lipid synthesis and storage within lipid droplets (LDs). Reduced hepatic glucose production in Hdac3-depleted liver is a result of the metabolic rerouting rather than due to inherently defective gluconeogenesis. The lipid-sequestering LDs-coating protein Perilipin 2 is markedly induced upon Hdac3 deletion and contributes to the development of both steatosis and improved tolerance to glucose. These findings suggest that the sequestration of hepatic lipids ameliorates insulin resistance, and establish Hdac3 as a pivotal epigenomic modifier that integrate signals from the circadian clock in regulation of hepatic intermediary metabolism.