Background: Infections occur frequently in nursing home residents, mainly pneumonia, UTI and SSTI, usually requiring empirical antimicrobial treatment in an hypo-immune population, which contribute to the increasing frequency of antimicrobial agent-resistant organisms in nursing homes. Patients and methods: We did oropharyngeal swabs of residents of long-term facilities to detect their potential contamination with resistant pathogens in three nursing homes in Bratislava. Results: Multi-resistant pathogens were recorded in total of 71 clients, but only 5 (10%) were positive for gram-negative bacteria, however, in all, multi-resistant bacteria/yeast were obtained. Conclusions: Monthly surveillance cultures, but also admission cultures, not only rectal, but also from respiratory tract are advisable.