For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
36
views
40
references
 Top references
cited by
23
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      0
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      ID30B – a versatile beamline for macromolecular crystallography experiments at the ESRF

      research-article
      Author(s): a , * , b , b , b , b , a , b , b , a , a , b , b , a , b , b , b , a , a , a , a , b , b , a , a , b , b
      Publication date (Electronic):
      Journal: Journal of Synchrotron Radiation
      Publisher: International Union of Crystallography
      Conference name: Includes papers presented at Q2XAFS2017 – the International Workshop on Improving Data Quality in XAFS Spectrometry, Diamond Light Source, UK, 14–15 August 2017
      Conference date:
      Keywords: ID30B, MD2S, REX rapid nozzle changer, FlexHCD, SPINEplus gripper, experimental phasing, automation

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          ID30B, a versatile macromolecular crystallography beamline at the ESRF, is presented.

          Abstract

          ID30B is an undulator-based high-intensity, energy-tuneable (6.0–20 keV) and variable-focus (20–200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group’s beamlines to be constructed and commissioned as part of the ESRF’s Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B ( http://www.esrf.eu/id30b).

          Related collections

          Most cited references40

          • Record: found
          • Abstract: found
          • Article: not found

          The PILATUS 1M detector.

          E Pohl, A. WAGNER, H Toyokawa … (2006)
          The PILATUS 1M detector is a hybrid pixel array detector with over one million pixels that operate in single photon counting mode. The detector, designed for macromolecular crystallography, is the largest pixel array detector currently in use at a synchrotron. It is a modular system consisting of 18 multichip modules covering an area of 21 cm x 24 cm. The design of the components as well as the manufacturing of the detector including the bump-bonding was performed at the Paul Scherrer Institute (PSI). The use of a single photon counting detector for protein crystallography requires detailed studies of the charge collection properties of the silicon sensor. The 18 modules are read out in parallel, leading to a full frame readout-time of 6.7 ms. This allows crystallographic data to be acquired in fine-varphi-slicing mode with continuous rotation of the sample. The detector was tested in several experiments at the protein crystallography beamline X06SA at the Swiss Light Source at PSI. Data were collected both in conventional oscillation mode using the shutter, as well as in a fine-varphi-slicing mode. After applying all the necessary corrections to data from a thaumatin crystal, the processing of the conventional data led to satisfactory merging R-factors of the order of 8.5%. This allows, for the first time, determination of a refined electron density map of a macromolecular biological crystal using a silicon pixel detector.
          Article 0 comments Cited 180 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            A compound refractive lens for focusing high-energy X-rays

            V. Kohn, I. Snigireva, B. Lengeler … (1996)
            Article 0 comments Cited 140 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Global indicators of X-ray data quality

              Manfred S. Weiss (2001)
              Article 0 comments Cited 135 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Conference
                Journal ID (nlm-ta): J Synchrotron Radiat
                Journal ID (iso-abbrev): J Synchrotron Radiat
                Journal ID (publisher-id): J. Synchrotron Rad.
                Title: Journal of Synchrotron Radiation
                Publisher: International Union of Crystallography
                ISSN (Print): 0909-0495
                ISSN (Electronic): 1600-5775
                Publication date Collection: 01 July 2018
                Publication date (Electronic): 27 June 2018
                Publication date PMC-release: 27 June 2018
                Volume: 25
                Issue: Pt 4 ( publisher-idID: s180400 )
                Pages: 1249-1260
                Affiliations
                [a ] European Molecular Biology Laboratory, Grenoble Outstation , 71 avenue des Martyrs, Grenoble 38042, France
                [b ] European Synchrotron Radiation Facility , 71 avenue des Martyrs, Grenoble 38043, France
                Author notes
                Correspondence e-mail: andrewmc@ 123456embl.fr
                Author information
                https://orcid.org/0000-0003-4478-0136
                https://orcid.org/0000-0003-0020-1104
                https://orcid.org/0000-0001-5030-0122
                https://orcid.org/0000-0003-4487-9558
                https://orcid.org/0000-0003-1285-2190
                https://orcid.org/0000-0002-5702-843X
                https://orcid.org/0000-0002-1539-7130
                https://orcid.org/0000-0002-6937-7039
                https://orcid.org/0000-0002-1878-6684
                Article
                Publisher ID: ig5055 Coden ID: JSYRES Publisher Item ID: S1600577518007166
                DOI: 10.1107/S1600577518007166
                PMC ID: 6038607
                PubMed ID: 29979188
                SO-VID: aca9ea0c-c8d0-4b2f-b894-b591e6d02eb0
                Copyright © © McCarthy et al. 2018
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

                Conference name: Includes papers presented at Q2XAFS2017 – the International Workshop on Improving Data Quality in XAFS Spectrometry, Diamond Light Source, UK, 14–15 August 2017
                Conference location:
                Conference date:
                History
                Date received : 06 March 2018
                Date accepted : 13 May 2018
                Page count
                Pages: 12
                Funding
                Funded by: Agence Nationale de la Recherche
                Award ID: ANR-10-INBS-05
                This work was funded by Agence Nationale de la Recherche grant ANR-10-INBS-05.
                Categories
                Subject: Beamlines

                ScienceOpen disciplines: Radiology & Imaging
                Keywords: id30b,md2s,rex rapid nozzle changer,flexhcd,spineplus gripper,experimental phasing,automation
                Data availability:
                ScienceOpen disciplines: Radiology & Imaging
                Keywords: id30b, md2s, rex rapid nozzle changer, flexhcd, spineplus gripper, experimental phasing, automation

                Comments

                Comment on this article