Most recently, silver nanoparticles due to antibacterial properties have been considered
in medical science. So the aim of the study was green synthesis of silver nanoparticles
using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity.
After collection, identification and extraction of Berberis vulgaris was performed
production of silver nanoparticles. In the study effect of parameters such as AgNO3
concentration (0.5, 1, 3, 10 mM), aqueous extract (3, 5, 10, 15, 30 mL) and contact
time (1, 2, 6, 12, 24 h) were investigated in the synthesis of nanoparticles and also
the antibacterial effect of these nanoparticles was studied on Escherichia coli and
Staphylococcus aureus bacteria by Disk diffusion test and Minimum Inhibitory Concentration
test (MIC). According to XRD results and analysis of TEM, nanoparticles have spherical
shapes and size of 30 to 70 nm. On the other hand antibacterial tests showed these
nanoparticles have more antibacterial activity more than other extracts. Result showed
the biosynthesis of silver nanoparticles using aqueous extract of Berberis vulgaris
is a clean, inexpensive and safe method that has not been used any toxic substance
and consequently does not side effects and this nanoparticles has a high antibacterial
activity.