Milad Babaei: Role: ConceptualizationRole: Formal analysisRole: InvestigationRole: Writing – review
& editing
Yuekun Liu: Role: ConceptualizationRole: Formal analysisRole: InvestigationRole: MethodologyRole:
Writing – review & editing
Shelly M. Wuerzberger-Davis: Role: ConceptualizationRole: Formal analysisRole: InvestigationRole: MethodologyRole:
Writing – review & editing
Ethan Z. McCaslin: Role: InvestigationRole: Methodology
Christopher J. DiRusso: Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole:
MethodologyRole: Writing – review & editing
Alan T. Yeo: Role: ConceptualizationRole: Methodology
Larisa Kagermazova: Role: ConceptualizationRole: Methodology
Shigeki Miyamoto: Role: ConceptualizationRole: Funding acquisitionRole: Writing – review & editing
Thomas D. Gilmore:
ORCID: http://orcid.org/0000-0002-1910-768X
Role: ConceptualizationRole: Funding acquisitionRole: InvestigationRole: MethodologyRole:
ResourcesRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
Hodaka Fujii: Role: Editor
Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS ONE
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
25
September
2019
Publication date Collection: 2019
Volume: 14
Issue: 9
Electronic Location Identifier: e0222588
Affiliations
[1
]
Department of Biology, Boston University, Boston, Massachusetts, United States of
America
[2
]
Department of Oncology, McArdle Laboratory for Cancer Research, University of Wisconsin
Carbone Cancer Center, University of Wisconsin, Madison, Wisconsin, United States
of America
Hirosaki University Graduate School of Medicine, JAPAN
Author notes
Competing Interests: The authors have declared that no competing interests exist.
Author information
Article
Publisher ID:
PONE-D-19-03328
DOI: 10.1371/journal.pone.0222588
PMC ID: 6760803
PubMed ID: 31553754
SO-VID: acfc613b-e911-440c-a7a9-2e71bc5b2620
Copyright © © 2019 Babaei et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
Funded by: Foundation for the National Institutes of Health (US)
Award ID: CA077474
Award Recipient
:
Shigeki Miyamoto
Funded by: funder-id http://dx.doi.org/10.13039/100000009, Foundation for the National Institutes of Health;
Award ID: GM117350
Award Recipient
:
ORCID: http://orcid.org/0000-0002-1910-768X
Thomas D. Gilmore
This research was supported by the National Institutes of Health (
www.nih.gov) grants CA077474 (to S.M.) and GM117350 (to T.D.G.). M.B., Y.L., L.K, and E.Z.M.
were supported by funds from the Boston University Undergraduate Research Opportunities
Program (
www.bu.edu/urop). The funders had no role in study design, data collection and analysis, decision
to publish, or preparation of the manuscript.
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Cloning
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Cloning
Subject:
Research and analysis methods
Subject:
Biological cultures
Subject:
Cell lines
Subject:
293T cells
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
RNA
Subject:
Guide RNA
Subject:
Research and Analysis Methods
Subject:
Specimen Preparation and Treatment
Subject:
Mechanical Treatment of Specimens
Subject:
Specimen Disruption
Subject:
Cell Disruption
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
Gene expression
Subject:
DNA transcription
Subject:
Biology and life sciences
Subject:
Molecular biology
Subject:
Molecular biology techniques
Subject:
Cloning
Subject:
DNA cloning
Subject:
Research and analysis methods
Subject:
Molecular biology techniques
Subject:
Cloning
Subject:
DNA cloning
Subject:
Biology and Life Sciences
Subject:
Biochemistry
Subject:
Proteins
Subject:
Post-Translational Modification
Subject:
Phosphorylation
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Molecular Biology Assays and Analysis Techniques
Subject:
Gene Expression and Vector Techniques
Subject:
Protein Expression
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Molecular Biology Assays and Analysis Techniques
Subject:
Gene Expression and Vector Techniques
Subject:
Protein Expression