Methodology: simplified preparation of a DNA ladder using PCR.

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Abstract

Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mixing. Fragments of 100- to 1000-bp DNA were successfully amplified; the sequences showed 100% identity with lambda DNA. This prepared DNA marker displayed clear bands, indicating that it can be used for molecular studies.

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Journal

Journal ID (iso-abbrev): Genet. Mol. Res.

Title: Genetics and molecular research : GMR

ISSN (Electronic): 1676-5680

ISSN (Print): 1676-5680

Publication date (Electronic): 2011

Volume: 10

Issue: 3

Affiliations

[1 ] Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Henan, P.R. China. wtianyuncn@126.com

Article

Publisher Item ID: gmr1177 Publisher Item ID: 10.4238/vol10-3gmr1177

PubMed ID: 21863555

SO-VID: ad0e2966-eb4c-49a5-bb0b-5f917be46346

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