A comparison of the protein content of different imaginal discs of Drosophila revealed that among more than 600 protein species that can be detected, only three show differences in concentration among different types of discs. Two of them form a doublet that can be resolved only by using extended electrophoresis conditions. This doublet was also reported to have a nonhomogeneous distribution within some discs. Here we show that these two proteins are tropomyosin components, and that they are associated not with the discs themselves but with a new type of muscle that connects some of the discs together.