Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Glycosyl-sn-1,2-dimyristylphosphatidylinositol is the membrane anchor for Trypanosoma equiperdum and T. (Nannomonas) congolense variant surface glycoproteins.

Molecular and Biochemical Parasitology

Animals, Electrophoresis, Polyacrylamide Gel, Glycolipids, analysis, Glycoproteins, Glycosylphosphatidylinositols, Myristic Acid, Myristic Acids, metabolism, Nitrous Acid, Phosphatidylinositols, Phospholipases, Trypanosoma, Trypanosoma brucei brucei, Variant Surface Glycoproteins, Trypanosoma, Trypanosoma congolense

Read this article at

ScienceOpenPubMed
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      We have analysed the structures of the Trypanosoma (Nannomonas) congolense and T. equiperdum variant surface glycoprotein (VSG) membrane anchors. Myristic acid uptake, phospholipase treatment, and nitrous acid deamination showed that, for each species, the anchor is glycosyl-sn-1,2-dimyristylphosphatidylinositol, as has been previously described for T. brucei. Osmotic lysis of these trypanosomes resulted in the release of soluble VSG, lacking fatty acid. In both species and in T. evansi, an endogenous phospholipase C, which cleaved diacylglycerol from membrane form VSG, was identified.

      Related collections

      Author and article information

      Journal
      2957588

      Comments

      Comment on this article