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      Increased Expression of Ecto-NOX Disulfide-thiol Exchanger 1 (ENOX1) in Diabetic Mice Retina and its Involvement in Diabetic Retinopathy Development

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          Abstract

          Background/Aim: Diabetic retinopathy (DR) is a type of retinal damage caused by a complication of diabetes and is a major cause of blindness in working-age adults. Ecto-NOX disulfide-thiol exchanger 1 (ENOX1) is a member of the ecto-NOX family involved in the plasma membrane electron transport pathway. This study aimed to investigate the role of ENOX1 in the development of DR. Materials and Methods: Human retinal endothelial cells (HRECs) and human retinal pigment epithelial cells (HREpiCs) exposed to a high concentration (25 mM) of D-glucose and type 2 diabetes (T2D) mice (+Lepr db /+Lepr db, db/db) with retinopathy were used as models to determine the ENOX1 expression levels there. Results: Our results showed that ENOX1 expression levels did not significantly change in both HRECs and HREpiCs under hyperglycemic conditions for 48 h. Nevertheless, ENOX1 expression increased significantly in T2D mouse retinas, particularl y in the photoreceptor layer, compared to the control mouse retinas. Conclusion: Different retinal ENOX1 expression in T2D mice and control mice suggested that ENOX1 may be involved in DR development.

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          Author and article information

          Journal
          In Vivo
          In Vivo
          In Vivo
          International Institute of Anticancer Research
          0258-851X
          1791-7549
          3 November 2019
          Nov-Dec 2019
          : 33
          : 6
          : 1801-1806
          Affiliations
          [1 ]School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C.
          [2 ]Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
          [3 ]Center for Translational Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.
          [4 ]Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan, R.O.C.
          [5 ]Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan, R.O.C.
          [6 ]Department of Anatomical Pathology, Taipei Institute of Pathology, Taipei, Taiwan, R.O.C.
          [7 ]Department of Public Health, China Medical University, Taichung, Taiwan, R.O.C.
          [8 ]Center for Personalized Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.
          [9 ]Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan, R.O.C.
          [10 ]Children’s Hospital of China Medical University, Taichung, Taiwan, R.O.C.
          [11 ]Department of Medical Genetics, China Medical University Hospital, Taichung, Taiwan, R.O.C.
          Author notes
          Fuu-Jen Tsai and Wen-Ling Liao, Department of Medical Research, China Medical University Hospital, No. 2, Yuh- Der Road, Taichung 404, Taiwan, R.O.C. Tel: + 886 422052121 (Ext. 2041), Fax: +886 422053425 d0704@ 123456www.cmuh.org.tw (F.J.T) and wl0129@ 123456mail.cmu.edu.tw (W.L.L)
          Article
          PMC6899154 PMC6899154 6899154
          10.21873/invivo.11671
          6899154
          31662505
          ad5d3a49-2e9c-4de2-9ee7-4e0bd1a7669e
          Copyright 2019, International Institute of Anticancer Research
          History
          : 23 July 2019
          : 4 September 2019
          Categories
          Research Article

          Type 2 diabetes,T2D mice,ENOX1,diabetic retinopathy
          Type 2 diabetes, T2D mice, ENOX1, diabetic retinopathy

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