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      Analyzing lysosomes in live cells.

      Methods in enzymology

      Rats, Protein Transport, physiology, Phagocytosis, metabolism, Lysosomes, analysis, Lysosome-Associated Membrane Glycoproteins, cytology, Kidney, Humans, HeLa Cells, Green Fluorescent Proteins, Fluorescent Dyes, Fibroblasts, Endocytosis, Dextrans, methods, Cell Tracking, Animals

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          Abstract

          Lysosomes are an important cellular organelle that receive and degrade macromolecules from the secretory, endocytic, autophagic, and phagocytic membrane-trafficking pathways. Defects in lysosome function lead to the development of disease with often-severe consequences to the individual. Since the discovery of lysosomes by Christian de Duve over 50 years ago, research into endocytic and lysosomal biology has allowed for the development of tools to understand further the role of lysosomes in cells. There are now several fluorescent probes that can be used to visualize and assess membrane traffic to the lysosome as well as probes to assess the activity of lysosomal hydrolases in live cells. This chapter describes the current methods used to measure lysosome function in live cells. Copyright © 2012 Elsevier Inc. All rights reserved.

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          Author and article information

          Journal
          10.1016/B978-0-12-388448-0.00016-4
          22289452

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