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      Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

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      Science
      American Association for the Advancement of Science (AAAS)

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          Abstract

          Visibly fluorescent proteins (FPs) from jellyfish and corals have revolutionized many areas of molecular and cell biology, but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extinction coefficient >90,000 M(-1) cm(-1); and quantum yield of 0.07. IFPs express well in mammalian cells and mice and spontaneously incorporate biliverdin, which is ubiquitous as the initial intermediate in heme catabolism but has negligible fluorescence by itself. Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering.

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          Author and article information

          Journal
          Science
          Science
          American Association for the Advancement of Science (AAAS)
          0036-8075
          1095-9203
          May 07 2009
          May 08 2009
          May 07 2009
          May 08 2009
          : 324
          : 5928
          : 804-807
          Article
          10.1126/science.1168683
          2763207
          19423828
          ada56e0f-721f-4511-a23e-a5393c1723c8
          © 2009
          History

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