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Girardinus is a Cuban genus of poeciliid fishes. It comprises 7 freshwater-restricted
species most of them inhabiting specific ecosystems or regions within the island.
Girardinus falcatus, the Gold-bellied Topminnow, is common in lowlands of western
and central Cuba but thought not to be present in the East. Herein we provide the
first wellsupported evidence of the occurrence of this species in eastern Cuba. Sixteen
specimens were collected and identified primarily by body shape and coloration, and
corroborated by gonopodial morphology and by sequences of the mitochondrial gene cytochrome