Foodborne pathogen is the primary cause of foodborne disease outbreak. Given its great
damage, a sensitive, simple and rapid detection method is demanded. Herein, we described
a self-priming polydimethylsiloxane (PDMS)/paper hybrid microfluidic chip, termed
SPH chip, with mixed-dye-loaded loop-mediated isothermal amplification (LAMP) for
multiplex foodborne pathogens detection. Staphylococcus aureus (SA) and Vibrio parahaemolyticus
(VP) were chosen to verify the novel method. Compared to other similar detection devices,
the SPH chip required easier fabrication process, less operation steps and lower cost.
Additionally, the reaction result, especially for the weak-positive reaction, could
be judged more accurately and conveniently due to the use of mixed-dye. Without pre-enrichment
of bacteria in the food contaminated sample, the limit of detection (LOD) reached
down to 1000 CFU mL-1 with high specific. Additionally, for fully exploiting the potential
of SPH chip, a conceptual eight-channel detection chip was also developed. Overall,
the reliable and excellent result demonstrated that the novel method had great potential
to be applied in the wider range of pathogens detection or disease diagnose, especially
in some resource-limited area.