37
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Refinement of tools for targeted gene expression in Drosophila.

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A wide variety of biological experiments rely on the ability to express an exogenous gene in a transgenic animal at a defined level and in a spatially and temporally controlled pattern. We describe major improvements of the methods available for achieving this objective in Drosophila melanogaster. We have systematically varied core promoters, UTRs, operator sequences, and transcriptional activating domains used to direct gene expression with the GAL4, LexA, and Split GAL4 transcription factors and the GAL80 transcriptional repressor. The use of site-specific integration allowed us to make quantitative comparisons between different constructs inserted at the same genomic location. We also characterized a set of PhiC31 integration sites for their ability to support transgene expression of both drivers and responders in the nervous system. The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication.

          Related collections

          Author and article information

          Journal
          Genetics
          Genetics
          Oxford University Press (OUP)
          1943-2631
          0016-6731
          Oct 2010
          : 186
          : 2
          Affiliations
          [1 ] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. pfeifferb@janelia.hhmi.org
          Article
          genetics.110.119917
          10.1534/genetics.110.119917
          2942869
          20697123
          ade065d1-e135-408a-b902-f42eebf4228d
          History

          Comments

          Comment on this article