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      Flow cytometry

      Review of Scientific Instruments
      AIP Publishing

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          Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining

          K Krishan (1975)
          A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.
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            Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence.

            A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.
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              Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

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                Author and article information

                Journal
                Review of Scientific Instruments
                Review of Scientific Instruments
                AIP Publishing
                0034-6748
                1089-7623
                September 1984
                September 1984
                : 55
                : 9
                : 1375-1400
                Article
                10.1063/1.1137948
                adf911d5-19be-4e09-acf3-71b0adb023b2
                © 1984
                History

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