Abstract. Objectives: To evaluate the protective effect of the chelating agent deferiprone (DFP) with and without taurine (Tau) against aluminum (Al)-induced liver toxicity. Method: 56 male Wistar rats were randomly divided into seven groups: control, Al-treated, low-dose DFP, high-dose DFP, Tau + low-dose DFP and Tau + high-dose DFP. The control group received 1 mL/kg/day saline solution for 8 weeks. The other groups were exposed to Al at a dose of 281.40 mg/kg/day orally for 4 weeks. Then, they were administered with 1 mL/kg/day saline solution, 400 mg/(kg×day) Tau, 13.82 mg/(kg×day) DFP, 27.44 mg/(kg×day) DFP, 400 mg/(kg×day) Tau +13.82 mg/(kg×day) DFP, and 400 mg/(kg×day) Tau +27.44 mg/(kg×day) DFP for 4 weeks. After that, the changes in markers of oxidative stress, activities of antioxidant enzymes, triphosphatase (ATPase) in the liver and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were determined. Al and iron (Fe), copper (Cu), zinc (Zn), calcium (Ca), and magnesium (Mg) were determined by atomic absorption spectrophotometry. Results: Serum ALT, AST, and liver malondialdehyde (MDA) were significantly higher after Al intake, while the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and ATPases were significantly lower compared with the control group (p < 0.05). Al and Cu concentrations in the liver were higher and Fe and Zn concentrations were lower in Al-treated rats (p < 0.05). Treatment with DFP or Tau alone restored MDA, Al, and Fe levels, and activities of GSH-Px, ALT, Na + K + -ATPase, and Mg 2+ -ATPase to control levels (p < 0.05). Combined DFP and Tau treatment was more effective than DFP or Tau alone (p < 0.05). Conclusion: Combined DFP and Tau treatment alleviated oxidative stress and improved liver function more effectively than DFP alone.
|ScienceOpen disciplines:||Endocrinology & Diabetes, General medicine, Medicine, Gastroenterology & Hepatology, Nutrition & Dietetics|
|Keywords:||aluminum, antioxidant system, DFP, ATPase, taurine|