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      Molecular database for classifying Shorea species (Dipterocarpaceae) and techniques for checking the legitimacy of timber and wood products

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          Abstract

          The extent of tropical forest has been declining, due to over-exploitation and illegal logging activities. Large quantities of unlawfully extracted timber and other wood products have been exported, mainly to developed countries. As part of the export monitoring effort, we have developed methods for extracting and analyzing DNA from wood products, such as veneers and sawn timbers made from dipterocarps, in order to identify the species from which they originated. We have also developed a chloroplast DNA database for classifying Shorea species, which are both ecologically and commercially important canopy tree species in the forests of Southeast Asia. We are able to determine the candidate species of wood samples, based on DNA sequences and anatomical data. The methods for analyzing DNA from dipterocarp wood products may have strong deterrent effects on international trade of illegitimate dipterocarp products. However, the method for analyzing DNA from wood is not perfect for all wood products and need for more improvement, especially for plywood sample. Consequently, there may be benefits for the conservation of tropical forests in Southeast Asia.

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          The online version of this article (doi:10.1007/s10265-010-0348-z) contains supplementary material, which is available to authorized users.

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          Universal primers for amplification of three non-coding regions of chloroplast DNA.

          Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked for most species tested including algae, bryophytes, pteridophytes, gymnosperms and angiosperms. The fact that they amplify chloroplast DNA non-coding regions over a wide taxonomic range means that these primers may be used to study the population biology (in supplying markers) and evolution (inter- and probably intraspecific phylogenies) of plants.
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            A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants.

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              Identification of refugia and post-glacial colonisation routes of European white oaks based on chloroplast DNA and fossil pollen evidence

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                Author and article information

                Contributors
                +81-29-8298261 , +81-29-8298261 , ytsumu@ffpri.affrc.go.jp
                Journal
                J Plant Res
                J. Plant Res
                Journal of Plant Research
                Springer Japan (Japan )
                0918-9440
                1618-0860
                15 May 2010
                15 May 2010
                January 2011
                : 124
                : 1
                : 35-48
                Affiliations
                [1 ]Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687 Japan
                [2 ]Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572 Japan
                [4 ]Faculty of Agriculture, Ehime University, Matsuyama, 790-8566 Japan
                [5 ]Center for Ecological Research, Kyoto University, Hirano-cho, Otsu, 520-2113 Japan
                [6 ]Forest Department Sarawak, Forest Research Center, Sarawak, 93660 Kuching, Sarawak Malaysia
                [7 ]Institute of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Göttingen, Germany
                [8 ]Faculty of Forestry, Gadjah Mada University, Bulaksumur, Yogyakarta, 55281 Indonesia
                [9 ]Forest Research Institute Malaysia, 52109 Kepong, Selangor Malaysia
                [10 ]Hayama Center for Advanced Studies, Graduate University for Advanced Studies, Hayama, Kanagawa 240-0193 Japan
                [11 ]Forestry Research Institute, Oji Paper Company Limited, 24-9 Nobono-cho, Kameyama, Mie 519-0212 Japan
                [12 ]Japan International Research Center for Agricultural Sciences, Ohwashi, Tsukuba, 305-8686 Japan
                Article
                348
                10.1007/s10265-010-0348-z
                3388261
                20473629
                ae3eb5e8-f21a-4e5b-8fc0-144b3104e719
                © The Botanical Society of Japan and Springer 2010
                Categories
                Regular Paper
                Custom metadata
                © The Botanical Society of Japan and Springer 2011

                Plant science & Botany
                database,conservation,illegal logging,tropical forest,southeast asia,cpdna
                Plant science & Botany
                database, conservation, illegal logging, tropical forest, southeast asia, cpdna

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