Journal ID (nlm-ta): F1000Res
Journal ID (iso-abbrev): F1000Res
Journal ID (pmc): F1000Research
Title:
F1000Research
Publisher:
F1000Research
(London, UK
)
ISSN
(Electronic):
2046-1402
Publication date
(Electronic):
31
May
2017
Publication date Collection: 2017
Volume: 6
Electronic Location Identifier: 760
Affiliations
[1
]University of California at Santa Cruz, Santa Cruz, CA, USA
[2
]Michael Smith Laboratories and Djavad Mowfaghian Centre for Brain Health, University
of British Columbia, Vancouver, Canada
[3
]School of Life Sciences, University of Nottingham, Nottingham, UK
[4
]Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
[5
]Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, UK
[6
]Malaghan Institute of Medical Research, Wellington, New Zealand
[7
]Norwich Medical School, University of East Anglia, Norwich, UK
[8
]Earlham Institute, Norwich Research Park, Norwich, UK
[9
]Science for Life Laboratory, IGP, Uppsala University, Uppsala, Sweden
[10
]ZF-screens B.V., Leiden, Netherlands
[11
]European Molecular Biology Laboratory (EMBL), European Bioinformatics Institute, Wellcome
Trust Genome Campus, Hinxton, UK
[12
]Virginia Commonwealth University, Richmond, VA, USA
[1
]Department of Population Health and Reproduction, University of California, Davis,
Davis, CA, USA
[2
]Department of Population Health and Reproduction, University of California, Davis,
CA, USA
[1
]Artificial Intelligence Research Center, National Institute of Advanced Industrial
Science and Technology (AIST), Tokyo, Japan
[2
]Department of Computational Biology and Medical Sciences, Graduate School of Frontier
Sciences, The University of Tokyo, Kashiwa, Japan
[3
]Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), National Institute
of Advanced Industrial Science and Technology (AIST), Tokyo, Japan
[1
]Department of Genetics, University Medical Center Utrecht, Utrecht, Netherlands
Author notes
MJ and JT coordinated the study. The MARC group collectively designed the study. ML,
SM, and JT performed the experiments. VZ, RL, ML, MJ, RL and CI ran data pre-processing
steps. MJ, CI and JT analysed the data. MJ and BB drafted the manuscript. All authors
participated in discussions relating to the generation and analysis of the data and
edited and approved the final manuscript for submission.
Competing interests: All flow cells and library preparation kits were provided by ONT free of charge. Ewan
Birney is a paid consultant of ONT. MJ, HEO, JT, ML, CI, HJ, JOG and BB have accepted
reimbursement for conference travel expenses from ONT. VZ was funded for his work
on this project from Oxford Nanopore through an agreement with EMBL.
Competing interests: No competing interests were disclosed.
Competing interests: No competing interests were disclosed.
Competing interests: No competing interests were disclosed.
Author information
Article
DOI: 10.12688/f1000research.11354.1
PMC ID: 5538040
PubMed ID: 28794860
SO-VID: aea9bd61-ebc4-4d08-afb7-36bf57945c3f
Copyright © Copyright: © 2017 Jain M et al.
License:
This is an open access article distributed under the terms of the Creative Commons
Attribution Licence, which permits unrestricted use, distribution, and reproduction
in any medium, provided the original work is properly cited.
Funded by: National Human Genome Research Institute
Award ID: HG007827
Funded by: The Michael Smith Foundation for Health Research and the Koerner Foundation
Funded by: Biotechnology and Biological Sciences Research Council
Award ID: BB/J010375/1
Funded by: National Science Foundation
Award ID: DEB-1355059
Funded by: National Human Genome Research Institute
Award ID: HG006321
Funded by: Canadian Institutes of Health Research
Award ID: 10677
Funded by: Brain Canada Multi-Investigator Research Initiative Grant with matching support from
Genome British Columbia
Funded by: Biotechnology and Biological Sciences Research Council
Award ID: BB/M020061/1
Funded by: Wellcome Trust
Award ID: 090532/Z/09/Z
Funded by: UK Antimicrobial Resistance Cross Council Initiative
Award ID: MR/N013956/1
Funded by: Rosetrees Trust
Award ID: A749
Funded by: E o R Börjessons Foundation
The following grants supported the research of the following authors: NHGRI, USA award
numbers HG006321 and HG007827 (MJ and HEO, UCSC), Canadian Institutes of Health Research
#10677 (JT and TS, UBC), Brain Canada Multi-Investigator Research Initiative Grant
with matching support from Genome British Columbia, the Michael Smith Foundation for
Health Research and the Koerner Foundation (JT and TS, UBC), BBSRC grant BB/M020061/1
(ML and SM, Nottingham), Wellcome Trust grant 090532/Z/09/Z (CI, WTCHG), UK Antimicrobial
Resistance Cross Council Initiative supported by the seven research councils (MR/N013956/1)
and Rosetrees Trust grant A749 (JOG, UEA), BBSRC grant BB/J010375/1 (RML, Earlham),
E o R Börjessons foundation (OW, Uppsala), and National Science Foundation DEB-1355059
(BB, VCU).