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      Speeding up three-dimensional protein NMR experiments to a few minutes.

      Journal of the American Chemical Society
      Nuclear Magnetic Resonance, Biomolecular, methods, Time Factors

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          Abstract

          We demonstrate for different protein samples that three-dimensional HNCO and HNCA correlation spectra may be recorded in a few minutes acquisition time using the band-selective excitation short-transient sequences presented here. This opens new perspectives for the NMR structural investigation of unstable protein samples and real-time site-resolved studies of protein kinetics.

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          Author and article information

          Journal
          16834371
          10.1021/ja062025p

          Chemistry
          Nuclear Magnetic Resonance, Biomolecular,methods,Time Factors
          Chemistry
          Nuclear Magnetic Resonance, Biomolecular, methods, Time Factors

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