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      Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis

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          Abstract

          Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3–10.0 µg·kg −1, with a limit of detection (LOD) of 0.1 µg·kg −1 and recoveries of 87.2%–114.3%, within 10 min. The results showed good correlation (R 2 > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg −1. The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.

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          Time-Resolution in Fluorometry Technologies, Labels, and Applications in Bioanalytical Assays

          Ilkka Hemmilä, Veli-Matti Mukkala (2008)
          Article 0 comments Cited 38 times – based on 0 reviews     Review now
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            A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products

            Marjan Majdinasab, Mahmoud Sheikh-Zeinoddin, Sabihe Soleimanian-Zad … (2015)
            Article 0 comments Cited 18 times – based on 0 reviews     Review now
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              Graphene oxide: an adsorbent for the extraction and quantification of aflatoxins in peanuts by high-performance liquid chromatography.

              Li Yu, Peiwu Li, Qi Zhang … (2013)
              In this paper, graphene oxide (GO) was synthesized and specifically selected by centrifugation to extract four aflatoxins (B1, B2, G1, and G2) as an effective adsorbent. Then, the amount of aflatoxins was quantitatively measured by high-performance liquid chromatography (HPLC). The GO was characterized by X-ray diffraction (XRD), atomic force microscopy (AFM), and ultraviolet (UV) spectrophotometer. Several parameters that could affect the extraction efficiency, including the GO amount, methanol concentration in the extraction solvent, spiked amount, extraction time, and elution cycle, were also investigated and optimized in this work. Under optimal conditions, good linear relationships were achieved with the correlation coefficient (r) ranging from 0.99217 to 0.99995. The detection limit of this method for the four aflatoxins ranged from 0.08 to 0.65ng/g. Finally, the proposed method has been successfully applied to determine aflatoxins in peanut samples. The results show that the recoveries of the four aflatoxins range from 85.1% to 100.8% with the relative standard deviations between 2.1% and 7.9%.
              Article 0 comments Cited 15 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Alexander Star: Role: Academic Editor
                Journal
                Journal ID (nlm-ta): Sensors (Basel)
                Journal ID (iso-abbrev): Sensors (Basel)
                Journal ID (publisher-id): sensors
                Title: Sensors (Basel, Switzerland)
                Publisher: MDPI
                ISSN (Electronic): 1424-8220
                Publication date (Electronic): 14 July 2016
                Publication date Collection: July 2016
                Volume: 16
                Issue: 7
                Electronic Location Identifier: 1094
                Affiliations
                [1 ]Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; wang416929@ 123456gmail.com (D.W.); zhangwen@ 123456oilcrops.cn (W.Z.)
                [2 ]Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
                [3 ]Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
                [4 ]Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China
                [5 ]Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China
                Author notes
                [* ]Correspondence: zwzhang@ 123456whu.edu.cn (Z.Z.); peiwuli@ 123456oilcrops.cn (P.L.); zhangqi01@ 123456caas.cn (Q.Z.); Tel.: +86-27-8681-2943 (P.L.); Fax: +86-27-8681-2862 (P.L.)
                [†]

                These authors contributed equally to this work.

                Article
                Publisher ID: sensors-16-01094
                DOI: 10.3390/s16071094
                PMC ID: 4970140
                PubMed ID: 27428975
                SO-VID: aeda9141-16b9-4824-a589-91d87bd3a69d
                Copyright © © 2016 by the authors; licensee MDPI, Basel, Switzerland.
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 01 May 2016
                Date accepted : 06 July 2016
                Categories
                Subject: Article

                ScienceOpen disciplines: Biomedical engineering
                Keywords: time-resolved fluorescent immunochromatographic strip,aflatoxin b1,soybean sauce,food safety
                Data availability:
                ScienceOpen disciplines: Biomedical engineering
                Keywords: time-resolved fluorescent immunochromatographic strip, aflatoxin b1, soybean sauce, food safety

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