First record of the genus Hennegoides Lom, Tonguthai and Dyková, 1991 from Punjab (Ind ia) infecting the catfish, Sperata seenghala (Sykes, 1839)

Abstract

Sperata seenghala (Sykes, 1839) the Giant river-catfish, is one of the largest freshwater catfish of Indian sub-continent and commonly called as singhara and seenghala. Catfish is a favourite food fish due to its palatability with high nutritional value. S. seenghala (25–27 cm in length) were procured from Chamkaur Sahib, a sub divisional town in the district of Rupnagar in the Indian State of Punjab. Prominent pale, thread-like plasmodia of Hennegoides seenghalae n. sp. were observed on the gills and histological examination located them in the epithelial lining of the gill filament (Intrafilamental epithelial type, FE). The prevalence was 32% (12 fish were infected out of 37 examined). The total myxospore length of He. seenghalae was 46.6 μm with myxospore body length 7.5 μm strongly vaulted from one side with caudal appendage length of 39.1 (25.5–45) μm. The myxospores were closely compared morphologically with five known species of the genus from Indo-Malayan region and another recently reported from the USA. Molecular analysis based on 18S rDNA sequence (1947bp) indicated 80%–91% sequence similarity with other myxozoan parasites ( Myxobolus, Henneguya, Hennegoides and Unicauda). The most closely related species was Hennegoides pangasii, and was placed with the present species in the same subclade. The present study is the first report the genus Hennegoides from India.

Graphical abstract

Highlights

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  A novel Hennegoides species infecting Sperata seenghala is described.

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  This is the first report of Hennegoides from India.

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  Hennegoides seenghalae n. sp. is characterized using a combination of morphological and molecular methods.