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      An Efficient Procedure for the Determination of Progesterone by Radioimmunoassay Applied to Bovine Peripheral Plasma

      , ,

      Hormone Research in Paediatrics

      S. Karger AG

      Radioimmunoassay, Progesterone, Bovine species

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          Abstract

          The radioimmunoassay described for the determination of progesterone only involves extraction of the plasma andsubsequent performance of the protein-binding reaction. The antisera used were obtained from rabbits and directed against 11α -OH-progesterone hemisuccinate BSA. A high degree of reliability was obtained for the criteria specificity, sensitivity, accuracy and precision. Application of the method during the bovine oestrous cycle revealed that there is no preovulatory rise of progesterone in this species. The presence of progesterone in peripheral plasma of bulls was demonstrated.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1973
          1973
          21 November 2008
          : 4
          : 5
          : 302-310
          Affiliations
          Institut für Physiologie, Technische Universität München-Weihenstephan, Freising
          Article
          178317 Horm Res 1973;4:302–310
          10.1159/000178317
          4796386
          © 1973 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
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          Paper

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