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      Glowing Podocytes in Living Mouse: Transgenic Mouse Carrying a Podocyte-Specific Promoter

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          Green fluorescence protein (GFP) has been utilized as a marker of gene expression due to the great advantage in its simple and quick detectability. We generated transgenic mice carrying a GFP cDNA under the control of a β-actin/β-globin promoter (CX promoter) and cytomegalovirus enhancer. The green luminescence derived from GFP was apparent in skeletal muscle, pancreas, heart and kidney, but not in other tissues. The GFP expression in the kidney was localized in podocytes. Moreover, in situ hybridization of GFP showed that the transcriptional level was highly active in the podocytes. These results suggested that the glowing green fluorescence would be a useful in vivo marker of podocyte in these transgenic lines in the physiological and pathophysiological state, and that the CX promoter could allow a podocyte-specific expression of a molecule of interest in kidney.

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          Most cited references 4

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          Efficient selection for high-expression transfectants with a novel eukaryotic vector

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            Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis.

             Min Wang,  T Hazelrigg (1994)
            Subcellular RNA localization in different cell types leads to asymmetric distribution of proteins in these cells. The localization of bicoid (bcd) messenger RNA to the anterior pole of the developing Drosophila oocyte gives rise in embryogenesis to a steep concentration gradient of the bcd protein, a transcription factor that activates expression of zygotic genes needed for anterior development. The exuperantia (exu) gene is necessary for this localization of bcd mRNA. Here we express a chimaeric gene encoding a fusion between the Acquorea victoria green fluorescent protein (GFP) and the exu protein (Exu) in female germ cells, and find that the fusion protein fluoresces strongly in both live and fixed cells during Drosophila oogenesis. The fusion protein rescues an exu null allele, restoring full fertility to females, and is expressed and localized in a temporal and spatial pattern similar to native Exu. The high sensitivity of the GFP tag provides important new details on the subcellular localization of Exu. The fusion protein is found in particles concentrated at ring canals, where transport occurs between nurse cells and the oocyte. Drugs such as colchicine and taxol that affect microtubule stability alter localization of the particles. We propose that the particles are ribonucleoprotein complexes or vesicles which transport bcd mRNA along microtubules and target it to the anterior oocyte cortex.
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              Expression vector system based on the chicken β-actin promoter directs efficient production of interleukin-5


                Author and article information

                Nephron Exp Nephrol
                Cardiorenal Medicine
                S. Karger AG
                February 1999
                14 January 1999
                : 7
                : 1
                : 63-66
                aFirst Department of Medicine, University School of Medicine and bGenome Information Research Center, Osaka University, Osaka, Japan
                20586 Exp Nephrol 1999;7:63–66
                © 1999 S. Karger AG, Basel

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                Page count
                Figures: 2, References: 12, Pages: 4
                Self URI (application/pdf):
                Technical Seminar:Cell Type-Specific Transgene Expression in Mice


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