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      Detection of Haemophilus influenzae by loop-mediated isothermal amplification (LAMP) of the outer membrane protein P6 gene.

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          Abstract

          It is difficult and time-consuming to distinguish Haemophilus influenzae from the genotypically similar Haemophilus parainfluenzae, which is a commensal of the human oral cavity. The novel nucleic acid amplification technique of loop-mediated isothermal amplification (LAMP), which amplifies DNA under isothermal conditions (63 degrees C) with high specificity, efficiency, and rapidity, was evaluated for H. influenzae detection. A H. influenzae-specific LAMP primer set was designed for the outer membrane protein P6 gene. Primer set specificity was validated using 4 Haemophilus spp. and 13 other species. Within 60 min, LAMP detected 100 or more copies of purified DNA with a sensitivity that was 10-fold higher than that of conventional PCR. This method can be used to differentiate H. influenzae from H. parainfluenzae strains. Thus, LAMP may represent a sensitive and reliable means of diagnosing H. influenzae infection.

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          Author and article information

          Journal
          Jpn. J. Infect. Dis.
          Japanese journal of infectious diseases
          1344-6304
          1344-6304
          Feb 2007
          : 60
          : 1
          Affiliations
          [1 ] Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
          Article
          17314429
          af5d8fec-2db2-42d2-ba3a-a2d93a5f56c2
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