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      Genome-Wide Investigation of Biofilm Formation in Bacillus cereus

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          ABSTRACT

          Bacillus cereus is a soil-dwelling Gram-positive bacterium capable of forming structured multicellular communities, or biofilms. However, the regulatory pathways controlling biofilm formation are less well understood in B. cereus. In this work, we developed a method to study B. cereus biofilms formed at the air-liquid interface. We applied two genome-wide approaches, random transposon insertion mutagenesis to identify genes that are potentially important for biofilm formation, and transcriptome analyses by RNA sequencing (RNA-seq) to characterize genes that are differentially expressed in B. cereus when cells were grown in a biofilm-inducing medium. For the first approach, we identified 23 genes whose disruption by transposon insertion led to altered biofilm phenotypes. Based on the predicted function, they included genes involved in processes such as nucleotide biosynthesis, iron salvage, and antibiotic production, as well as genes encoding an ATP-dependent protease and transcription regulators. Transcriptome analyses identified about 500 genes that were differentially expressed in cells grown under biofilm-inducing conditions. One particular set of those genes may contribute to major metabolic shifts, leading to elevated production of small volatile molecules. Selected volatile molecules were shown to stimulate robust biofilm formation in B. cereus. Our studies represent a genome-wide investigation of B. cereus biofilm formation.

          IMPORTANCE In this work, we established a robust method for B. cereus biofilm studies and applied two genome-wide approaches, transposon insertion mutagenesis and transcriptome analyses by RNA-seq, to identify genes and pathways that are potentially important for biofilm formation in B. cereus. We discovered dozens of genes and two major metabolic shifts that seem to be important for biofilm formation in B. cereus. Our study represents a genome-wide investigation on B. cereus biofilm formation.

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          Author and article information

          Contributors
          Role: Editor
          Journal
          Appl Environ Microbiol
          Appl. Environ. Microbiol
          aem
          aem
          AEM
          Applied and Environmental Microbiology
          American Society for Microbiology (1752 N St., N.W., Washington, DC )
          0099-2240
          1098-5336
          21 April 2017
          16 June 2017
          1 July 2017
          : 83
          : 13
          : e00561-17
          Affiliations
          [a ]Department of Biology, Northeastern University, Boston, Massachusetts, USA
          [b ]Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China
          [c ]Institute of Biotechnology, Zhejiang University, Hangzhou, China
          [d ]Engineering Center of Bioresource Pesticide in Jiangsu Province, Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing, China
          University of Minnesota
          Author notes
          Address correspondence to Jian-hua Guo, jhguo@ 123456njau.edu.cn , or Yunrong Chai, y.chai@ 123456northeastern.edu .

          Citation Yan F, Yu Y, Gozzi K, Chen Y, Guo J, Chai Y. 2017. Genome-wide investigation of biofilm formation in Bacillus cereus. Appl Environ Microbiol 83:e00561-17. https://doi.org/10.1128/AEM.00561-17.

          Article
          PMC5478996 PMC5478996 5478996 00561-17
          10.1128/AEM.00561-17
          5478996
          28432092
          afde2505-222b-4e20-be9a-0f7f5eda8108
          Copyright © 2017 American Society for Microbiology.

          All Rights Reserved.

          History
          : 8 March 2017
          : 12 April 2017
          Page count
          supplementary-material: 3, Figures: 6, Tables: 4, Equations: 0, References: 54, Pages: 18, Words: 11482
          Funding
          Funded by: National Natural Science Foundation of China (NSFC) https://doi.org/10.13039/501100001809
          Award ID: 31471812
          Award ID: 31672075
          Award Recipient : Jian-hua Guo
          Funded by: National Natural Science Foundation of China (NSFC) https://doi.org/10.13039/501100001809
          Award ID: 31301707
          Award Recipient : Yun Chen
          Funded by: National Science Foundation (NSF) https://doi.org/10.13039/100000001
          Award ID: CAREER_1651732
          Award Recipient : Yunrong Chai
          Funded by: Northeastern University (NEU) https://doi.org/10.13039/501100004184
          Award ID: Start up grant
          Award Recipient : Yunrong Chai
          Funded by: Northeastern University (NEU) https://doi.org/10.13039/501100004184
          Award ID: Provost Research Fund
          Award Recipient : Kevin Gozzi
          Categories
          Environmental Microbiology
          Custom metadata
          July 2017

          Bacillus cereus ,biofilm formation,transcriptome,transposon mutagenesis

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