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      Isolation, Culture and Characterization of Human Renal Proximal Tubule and Collecting Duct Cells

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          The complexity and heterogeneity of the human nephron with regard to cell types make well-defined in vitro systems of renal cells valuable for studies of the pathogenetic mechanisms involved in nephrotoxicity. In our laboratory renal proximal tubule cells (PTC) and collecting duct cells (CDC) have been isolated, cultured and characterized from cadaver kidneys (postmortem time <24 h) for use in studies of renal cytotoxicity induced by therapeutics and bacteria. PTC seeded at 10<sup>6</sup> cells/ml formed confluent monolayers within 7 days. Histochemical markers were used to determine the origin of the cell cultures. Cells were negative for factor VIII, positive for cytokeratin and γ-glutamyltransferase (GGT), and bound winged pea lectin. CDC, isolated from the renal papillae, formed monolayers within 14 days of seeding. CDC were negative for factor VIII and GGT, positive for cytokeratin and bound peanut lectin. PTC and CDC isolates and cultures exhibited typical epithelial cell ultrastructure: cell junctions, intermediate filaments, microvilli, and numerous mitochondria. The morphological and histochemical evidence confirms that PTC and CDC can be isolated and cultured for use in in vitro studies.

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          γ-Glutamyltransferase in kidney brush border membranes

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            Effect of gentamicin on the lysosomal system of cultured human proximal tubular cells

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              The activities of the key enzymes of the γ-glutamyl cycle in microdissected segments of the rat nephron


                Author and article information

                Nephron Exp Nephrol
                Cardiorenal Medicine
                S. Karger AG
                December 1999
                28 October 1999
                : 7
                : 5-6
                : 353-359
                University of Maryland, Baltimore, Md., USA
                20633 Exp Nephrol 1999;7:353–359
                © 1999 S. Karger AG, Basel

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                Page count
                Figures: 4, References: 44, Pages: 7
                Self URI (application/pdf): https://www.karger.com/Article/Pdf/20633


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