For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
80
views
39
references
 Top references
cited by
96
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      981
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: not found

      Cell-Type-Specific TEV Protease Cleavage Reveals Cohesin Functions in Drosophila Neurons

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Summary

          Cohesin is a highly conserved multisubunit complex that holds sister chromatids together in mitotic cells. At the metaphase to anaphase transition, proteolytic cleavage of the α kleisin subunit (Rad21) by separase causes cohesin's dissociation from chromosomes and triggers sister-chromatid disjunction. To investigate cohesin's function in postmitotic cells, where it is widely expressed, we have created fruit flies whose Rad21 can be cleaved by TEV protease. Cleavage causes precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells, but not disaggregation of polytene chromosomes in salivary glands. Crucially, cleavage in postmitotic neurons is lethal. In mushroom-body neurons, it causes defects in axon pruning, whereas in cholinergic neurons it causes highly abnormal larval locomotion. These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa.

          Related collections

          Most cited references39

          • Record: found
          • Abstract: found
          • Article: not found

          Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.

          Christine Michaelis, Rafal Ciosk, Kim Nasmyth (1997)
          Cohesion between sister chromatids opposes the splitting force exerted by microtubules, and loss of this cohesion is responsible for the subsequent separation of sister chromatids during anaphase. We describe three chromosmal proteins that prevent premature separation of sister chromatids in yeast. Two, Smc1p and Smc3p, are members of the SMC family, which are putative ATPases with coiled-coil domains. A third protein, which we call Scc1p, binds to chromosomes during S phase, dissociates from them at the metaphase-to-anaphase transition, and is degraded by the anaphase promoting complex. Association of Scc1p with chromatin depends on Smc1p. Proteins homologous to Scc1p exist in a variety of eukaryotic organisms including humans. A common cohesion apparatus might be used by all eukaryotic cells during both mitosis and meiosis.
          Article 0 comments Cited 415 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons.

            T Kitamoto (2001)
            Behavior is a manifestation of temporally and spatially defined neuronal activities. To understand how behavior is controlled by the nervous system, it is important to identify the neuronal substrates responsible for these activities, and to elucidate how they are integrated into a functional circuit. I introduce a novel and general method to conditionally perturb anatomically defined neurons in intact Drosophila. In this method, a temperature-sensitive allele of shibire (shi(ts1)) is overexpressed in neuronal subsets using the GAL4/UAS system. Because the shi gene product is essential for synaptic vesicle recycling, and shi(ts1) is semidominant, a simple temperature shift should lead to fast and reversible effects on synaptic transmission of shi(ts1) expressing neurons. When shi(ts1) expression was directed to cholinergic neurons, adult flies showed a dramatic response to the restrictive temperature, becoming motionless within 2 min at 30 degrees C. This temperature-induced paralysis was reversible. After being shifted back to the permissive temperature, they readily regained their activity and started to walk in 1 min. When shi(ts1) was expressed in photoreceptor cells, adults and larvae exhibited temperature-dependent blindness. These observations show that the GAL4/UAS system can be used to express shi(ts1) in a specific subset of neurons to cause temperature-dependent changes in behavior. Because this method allows perturbation of the neuronal activities rapidly and reversibly in a spatially and temporally restricted manner, it will be useful to study the functional significance of particular neuronal subsets in the behavior of intact animals. Copyright 2001 John Wiley & Sons, Inc.
            Article 0 comments Cited 246 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.

              Vincent Guacci, Douglas Koshland, Alexander Strunnikov (1997)
              The S. cerevisiae MCD1 (mitotic chromosome determinant) gene was identified in genetic screens for genes important for chromosome structure. MCD1 is essential for viability and homologs are found from yeast to humans. Analysis of the mcd1 mutant and cell cycle-dependent expression pattern of Mcd1p suggest that this protein functions in chromosome morphogenesis from S phase through mitosis. The mcd1 mutant is defective in sister chromatid cohesion and chromosome condensation. The physical association between Mcd1p and Smc1p, one of the SMC family of chromosomal proteins, further suggests that Mcd1p functions directly on chromosomes. These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link.
              Article 0 comments Cited 226 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Kim Nasmyth
                Journal
                Journal ID (nlm-ta): Dev Cell
                Title: Developmental Cell
                Publisher: Cell Press
                ISSN (Print): 1534-5807
                ISSN (Electronic): 1878-1551
                Publication date PMC-release: 12 February 2008
                Publication date (Print): 12 February 2008
                Volume: 14
                Issue: 2
                Pages: 239-251
                Affiliations
                [1 ]Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
                [2 ]Institute of Zoology, University of Zurich, 8057 Zurich, Switzerland
                [3 ]Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
                [4 ]Stanford University, Department of Biological Sciences, Stanford, CA 94305, USA
                [5 ]Institute of Molecular Pathology, 1030 Vienna, Austria
                Author notes
                [∗ ]Corresponding author kim.nasmyth@ 123456bioch.ox.ac.uk
                [6]

                These authors contributed equally to this work.

                Article
                Publisher ID: DEVCEL1398
                DOI: 10.1016/j.devcel.2007.12.009
                PMC ID: 2258333
                PubMed ID: 18267092
                SO-VID: b0097e80-a5a2-4140-95c9-c1fb7d851d09
                Copyright © © 2008 ELL & Excerpta Medica.
                License:

                This document may be redistributed and reused, subject to certain conditions.

                History
                Date received : 5 November 2007
                Date revision received : 7 December 2007
                Date accepted : 12 December 2007
                Categories
                Subject: Article

                ScienceOpen disciplines: Developmental biology
                Keywords: cellcycle,molneuro,devbio
                Data availability:
                ScienceOpen disciplines: Developmental biology
                Keywords: cellcycle, molneuro, devbio

                Comments

                Comment on this article

                scite_

                Similar content981

                See all similar

                Cited by92

                See all cited by

                Most referenced authors808

                See all reference authors