Gynodian-Depot® is well suited for treatment of the characteristic symptoms accompanying the menopause in women. The plasma levels of prasterone and 17β-estradiol after intramuscular injection of Gynodian-Depot in women were studied using a radioimmunological method. The maximum active ingredient concentrations were found in the plasma of the subjects only a few days after administration. The depot action lasted on an average 14 days (17β-estradiol) and 18 days (prasterone) after administration, respectively. Prasterone enanthate is completely hydrolysed into the free steroid and the fatty acid.