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Abstract
One-dimensional Fourier-transform proton nuclear magnetic resonance (1H-NMR) spectroscopy
can be used to study biotransformations in situ, in vivo and in aqua (1H2O). Although
an insensitive method, it rapidly provides solution-structural information of mixtures
of diverse compounds that are used and formed during enzymic reactions and culture
fermentations; the samples do not require any physical or chemical processing for
analysis. The absolute stereochemistry of some reactions can also be determined, and
assessments of metabolic fluxes made. This technique, with appropriate modifications,
is of obvious value for on-line assessments of industrial fermentation processes.